FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_H11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_H11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001702
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAAAGAGCTTAAGGGAAATATGGTAGAAGGAATTGGTAAAAAGAAG23910.23869374324899023No Hit
GTATATATAGCTACAGTACCTCGATAAGAAGCAGTTAAGTGGTGAGACTG13090.130677586747356No Hit
CTTTATATCTCTCCATTAAGCTCTTTATTTATTTTACTAATTCATTTGAA11360.11340698131779711No Hit
GTCATTGATTAGTCCCAGTTTTTATTTGATTGGTGGCAGCATTTTTCTTT10600.1058198945394938No Hit
ATATTAACCAACTAGTGTTAATTTTTTAAGGTTATGGCTTTGTGTAGAAG10080.10062872990170728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG100.0070832255145.052281
AGCGAAA3900.089.262951
GCGAAAG5350.066.4191742
CGAGCGA1353.6379788E-1253.704292145
TGCCGAT1301.3096724E-1050.1903539
GCGGTAT1351.0262738E-842.961299
CGAAAGC8650.042.7568053
GATATAA10250.041.7467581
TCTCCGC1157.14218E-637.826504145
TATAAAG13300.037.622593
GTGCCGA1752.4301698E-937.2842648
TAGGGCG1803.1923264E-936.259455
ATCCTAT1501.130702E-633.845531
GTTATAA1801.2883174E-732.233841
ATATAAA13650.031.3451632
TAAGGTG2101.436274E-831.0795275
TAAAGAG16350.031.0478465
GTCTTAG3800.030.5373211
GAGCGAA1204.0494878E-430.2192271
GTATAGA4450.029.3364161
Produced by FastQC (version 0.11.5)