FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_H10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_H10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475031
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAACACCGCTCATGGATTATTCATGACACTAGCCAGACTCCGAGCTC21150.445234100511335No Hit
GTCTCATATCTATCCCTGTCTCTCTCTTTGTCTCTCACACTCTCTGTCTG19250.4052367108672908No Hit
CTGTAACACTGTGGTGGTTTCCACAGCAACGGCCCAGAGACAGAGGGTGA18280.38481699089112076No Hit
CCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCAAAGCTCAAGT14830.3121901518006193No Hit
ATTCATGACACTAGCCAGACTCCGAGCTCAACCAGAGGGCACAAACAATT14440.30398016129473654No Hit
GTACAGGAATGAGGAATGGATGCTGGAATATTTGTCTCCCCCTCCTCTGC13150.27682403885220125No Hit
TATCTATCCCTGTCTCTCTCTTTGTCTCTCACACTCTCTGTCTGTCTCAC12320.2593514949550661No Hit
ATCTATCCCTGTCTCTCTCTTTGTCTCTCACACTCTCTGTCTGTCTCACA12200.25682534403017904No Hit
ATAAAATATTTTCCAGTAATAAAATGCAACACCGCTCATGGATTATTCAT12030.2532466302199225No Hit
CACACACACCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCAAA11930.2511415044491833No Hit
GGCTTAATTAACATATAAAATATTTTCCAGTAATAAAATGCAACACCGCT10050.21156513995928689No Hit
CTATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCA10000.21051257707391727No Hit
GTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGA9860.20756540099488244No Hit
GAGTAATAGTAGCGTGTGTGTGTGTGTGTCTGTGTGTCAGTTATCAGATT9430.198513360180704No Hit
CACACACACACCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCA8930.18798773132700813No Hit
CTCATGGATTATTCATGACACTAGCCAGACTCCGAGCTCAACCAGAGGGC8380.17640953958794267No Hit
CACACACACACACCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGT8300.17472543897135134No Hit
GACAGAGAGTGAGAGACAGACAGGAAGTGAGAGACAGGGAGTGAGAGACA7450.15683186992006837No Hit
GAGTAGGAGTGACGTGGAAGCAGGCATCGGAGCAGGTGCAGGAGGTGGGA7230.15220059322444218No Hit
ATGTTATTTCTACCCTCATTTGACAGATTGGGACTTAGAGGTTTAGAAAT7060.14862187941418561No Hit
GATCAAACCATGTTGTTGGTAATGGCTTAATTAACATATAAAATATTTTC7050.1484113668371117No Hit
CAATTATGCCTCACTGAGTAATAGTAGCGTGTGTGTGTGTGTGTCTGTGT6980.14693777879759426No Hit
ATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATG6910.14546419075807684No Hit
CTATAGGAGACTGTAAGCCGCAGCTGAATCTGGGCTCCTCTGTGTGAGGC6900.14525367818100293No Hit
GGATTATTCATGACACTAGCCAGACTCCGAGCTCAACCAGAGGGCACAAA6800.14314855241026375No Hit
CTTTATAATCTTCGCCAGGGACTTCAGAAGCCTGTGTCACGTAGTAATTT6570.13830676313756365No Hit
GTAATAAAATGCAACACCGCTCATGGATTATTCATGACACTAGCCAGACT6550.1378857379834158No Hit
ACACTAGCCAGACTCCGAGCTCAACCAGAGGGCACAAACAATTATGCCTC6430.1353595870585288No Hit
CACATACACATGCACACACACATACACACACACACACACACACACACCGT6300.13262292355656788No Hit
ATCTGTAACACTGTGGTGGTTTCCACAGCAACGGCCCAGAGACAGAGGGT6170.12988626005460696No Hit
GTGTAGGAATGGGTGTGAGCGTAGGAGGTGGGAACAGGCAGGGGAGCCGG6070.12778113428386778No Hit
GTGCAGGAACTGGATCAGGTGCAGGAATAGGAGCAAGAACAGGAGCAGGT5960.1254654959360547No Hit
GTTGGTAATGGCTTAATTAACATATAAAATATTTTCCAGTAATAAAATGC5960.1254654959360547No Hit
ATATTTTCCAGTAATAAAATGCAACACCGCTCATGGATTATTCATGACAC5920.12462344562775902No Hit
CATCAAGAGGTTCTGTGATGCTGGGCAATTCATCTAAGCCTTAATTTGCT5900.1242024204736112No Hit
ATAGTAGCGTGTGTGTGTGTGTGTCTGTGTGTCAGTTATCAGATTATGCG5900.1242024204736112No Hit
TTCCATACCCGGGTGAAGAGGGATGTAGTGGGCGCTTGAGCAGAGGAGAG5870.12357088274238945No Hit
CATACACGCACACACATACACATGCACACACACATACACACACACACACA5840.1229393450111677No Hit
GTAACACTGTGGTGGTTTCCACAGCAACGGCCCAGAGACAGAGGGTGAGA5770.12146575697165028No Hit
CATAAGCACACACACTCACACTTAAGATGTCAGAAATGGCTGTGGATAGA5720.12041319408628069No Hit
GTCAAGTACAGAGTAAAATGTGGACTCTGGGATGGTACTTCTCAGTTGTA5620.1183080683155415No Hit
GCCTCAACCAGGGTCTCGAGAGGAGCCTTTACCTGGACTTCTTTGTGGCT5570.11725550543017192No Hit
CACATACACGCACACACATACACATGCACACACACATACACACACACACA5280.11115064069502834No Hit
GTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCAAAGCTCAAGTTG5200.10946654007843698No Hit
GTTAATGAGTTTTTAAAAAGCCTTCCAAAGTGATTATAAGATCCTTTTTT5170.10883500234721523No Hit
ACCATGTTGTTGGTAATGGCTTAATTAACATATAAAATATTTTCCAGTAA5170.10883500234721523No Hit
GGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATGTTGGG5130.10799295203891957No Hit
GTTGGGAACCATCTGTCAGATCATCCAGCAAAACACGCTGACATCCTTCA5130.10799295203891957No Hit
TCTTTGTGGCTCTTGCCATCTGTAACACTGTGGTGGTTTCCACAGCAACG5130.10799295203891957No Hit
GTACACAGAAAAGCAAATTAGAGAAACAGGATGTAAAGCACAATTCTATT5060.10651936399940214No Hit
ATAATAGCCTTGTGTGGTGACAAATGGTAGCCACACTTGCGGTGGGCATA5060.10651936399940214No Hit
TCATTAAGGTGCAGTAGACACAGGGTGACAGTTGTTGTTGATACGTCTTT4970.10462475080573688No Hit
CACTTAAGATGTCAGAAATGGCTGTGGATAGATTTTGCACGGCATAGTGT4950.10420372565158906No Hit
GTTGTTGGTAATGGCTTAATTAACATATAAAATATTTTCCAGTAATAAAA4910.1033616753432934No Hit
ATGTTGTTGGTAATGGCTTAATTAACATATAAAATATTTTCCAGTAATAA4800.1010460369954803No Hit
CCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAG4780.10062501184133248No Hit
TTATTTACCCAAGTGTGTAGGTCTGCTTTTAGATGGGAAGGGAGATACTG4770.10041449926425855No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG351.900844E-9124.28383145
AGCGAAA3200.0117.835511
AAGTATT451.2312266E-680.5628054
AGTATTG451.2312266E-680.5628055
TCATATC4150.075.127254
GTCTCAT4400.072.514161
GACTTAT502.3045832E-672.49889145
CTCATAT4750.068.690393
TCTCATA4750.065.637482
CATATCT4900.063.6281785
ATATCTA4950.062.9854666
GGAGTCC1400.056.963428
GACAAGT651.0915017E-555.780131
CAAGTAT651.0921827E-555.7742543
CTATTTG2900.055.0107421
TATTTGA2950.054.0726662
GTATTGG858.902807E-751.186471
GTTCACC1004.6968125E-850.7492267
GGACATC450.00962861248.3427731
AACGTCT450.00963261848.3376855
Produced by FastQC (version 0.11.5)