FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990084
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAAAGAGCTTAAGGGAAATATGGTAGAAGGAATTGGTAAAAAGAAG23640.2387676197171149No Hit
GTATATATAGCTACAGTACCTCGATAAGAAGCAGTTAAGTGGTGAGACTG12960.13089798441344372No Hit
CTTTATATCTCTCCATTAAGCTCTTTATTTATTTTACTAATTCATTTGAA11270.11382872564348076No Hit
GTCATTGATTAGTCCCAGTTTTTATTTGATTGGTGGCAGCATTTTTCTTT10530.10635461233592303No Hit
ATATTAACCAACTAGTGTTAATTTTTTAAGGTTATGGCTTTGTGTAGAAG10030.10130453577676239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC353.437462E-582.8561559
AGCGAAA3400.076.775431
GCGAAAG5200.050.1967852
GGTATAG700.001055910141.434361
CCGTTAG1053.8243943E-641.428089
AGCGCGT700.001056697641.428078145
GATATAA9400.035.483681
GGAACTT5550.033.966996
TATAAAG12400.033.9143143
TGCCGAT1551.4651232E-632.7415479
GTCTTAG3950.031.2068881
CGAAAGC8700.030.8360823
TTATATC7300.030.7904193
GTAGTAC950.004725211330.529043
GTATAGA4350.030.004191
ACACTAC4150.029.6984449
AACTTGC6200.029.2335248
CTTCCGG1000.006074779728.999655145
ATATAAA12150.028.6445312
TAAAGAG15050.028.424465
Produced by FastQC (version 0.11.5)