FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGAACAAAAATCAAAAAACAAAAAAAAGACAACAAAAAGAAAAAAAAA11.9230769230769231No Hit
GGAGCTAAGCGAGCAATTGAGCTCAGAGGCCTCATTTGAAAGGATTGAGA11.9230769230769231No Hit
ATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTC11.9230769230769231No Hit
TACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCT11.9230769230769231No Hit
TCGTGAAGGTCGCCCATTCTCATCCCTCTTTCTCCAAGCGAATCTCTGTA11.9230769230769231No Hit
GAAAACAAAACCAACAAAAATGAAGGCAATACTAGTAGTTCTGCTATATA11.9230769230769231No Hit
GAGACACAAATAGGTGCCATAGGGGAGACACACAAATTCAGCCGAGAAGA11.9230769230769231No Hit
GCTTTTATTACATAACATCCTTAAATAATGGAGAATTGTTTGGGTTTTTT11.9230769230769231No Hit
GCGCAAGCAGGGTGCCTACAACATGATGGAGTCCAACAACATGTCTAGGT11.9230769230769231No Hit
AATATATTAAAAAAGTACAGAATAAAACAACTCATAAATATAATCACTCA11.9230769230769231No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAACGCT11.9230769230769231No Hit
AGGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGACTATGTTAGGCTC11.9230769230769231No Hit
TTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGT11.9230769230769231No Hit
GTTCACAGCAGTAGGTAAAGAGTTCCACCACCTGGAAAAAAGAATAGAGA11.9230769230769231No Hit
GAATTTACTTTGTTAGTAATCTCGACAATGGCATTCTGTGTGCTCTTAAG11.9230769230769231No Hit
GGTGGAAAGCTTTCCTCTTGGAATCACTTTCTTTCCTCTTATGAAACTTG11.9230769230769231No Hit
CCCACTGCCCCTTTTTCGGGGACATAAATTCCAATTGACATACAATTGAT11.9230769230769231No Hit
GTAATGGACAGGCCTCATACAAGATATTAAGAATAGAAAAGGGAAAGATA11.9230769230769231No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC11.9230769230769231No Hit
GTAACATGGTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCC11.9230769230769231No Hit
CCCAGGTACCCTTTCCCGAGTCTTGCCATTTTTGTTGATAACATTATTGG11.9230769230769231No Hit
AATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAAC11.9230769230769231No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT11.9230769230769231No Hit
GTGCTGGTCACTAACCAAAGTGCTTAAAGATTTTCGTGGCTCTTAGGCTC11.9230769230769231No Hit
GTTAGTGGATGGGCTATATAAAGTAAAGACAAAAGTGTAAGAATCGGTTC11.9230769230769231No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG11.9230769230769231No Hit
CACTGAGCTCAATTGCTCTCTTAGCTCCTCATAATCGATGAAATCTCCTG11.9230769230769231No Hit
CCTGGTCAAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATG11.9230769230769231No Hit
GTGATGCTGTGCCATCTATTAGAAGAGGACTTATTTTCTCAATTTTCTTC11.9230769230769231No Hit
GGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGAATCGAACACAACAA11.9230769230769231No Hit
GATATGTGGCATCATTCAGGTTGGAATGCCAAATCATGATATTAGTAAGA11.9230769230769231No Hit
TAGATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGCCCGAGTT11.9230769230769231No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTACATCGGGCAAAGGAGGT11.9230769230769231No Hit
AACATGAGCAAAAAGAAGTCCTATATAAATAAGACAGGGACATTTGAATT11.9230769230769231No Hit
CCCCTAATTATCACTATGAGGAAAGCTCCTGTTATCCTGATTCTAGTGAA11.9230769230769231No Hit
CGTGTAATTGCTGCGCTTGCGGTATGGAAGGGCGCCCCTGAGCATCACAG11.9230769230769231No Hit
GGATCGAGTGACCAGGCAGCGGAGGCCATGGAGGTTGCTAATCAGACTAG11.9230769230769231No Hit
GGCGCAAAACAAAAGATGCTGGATCAGACCGAGTGATGGTGTCCCCTCTG11.9230769230769231No Hit
GAGAATACCATGGCCAAAATTATTGCCGCAGCAATTGACTGCTCGTCTCT11.9230769230769231No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGATCCATGGGGCCAAGGAGGT11.9230769230769231No Hit
ACATATATCACCAGAAAACAACCCCACACCTACAAAAACTTCCTTAGCAT11.9230769230769231No Hit
GCTGAATCTAACTAGCCCCAAAAGGGGACAGAAAAGAGAAGAAGGGTGGA11.9230769230769231No Hit
CTCTTGAGGCATTTCTTAATAGAGTCAAAACTGGCATTGCCTAAATGGTG11.9230769230769231No Hit
CTATCATCCAGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGCAA11.9230769230769231No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC11.9230769230769231No Hit
AGAGTCAATTGGTGTGGATGGCATGCCACTCTGCTGCATTTGAAGATTTA11.9230769230769231No Hit
TGGAGGTTTACACATCACGCCTGGAAGGAGTTAAATAATTAATTACCAAA11.9230769230769231No Hit
CTTTATTTATTTTACTAATTCATTTGAAATCATGGCTAAGTCATTGATTA11.9230769230769231No Hit
GTCTTCTAACCGAGGTCGAAACGTACGATCTTTCTATCATCCAGTCAGGC11.9230769230769231No Hit
GAATGATGCCACATATCAGAGAACAAGCGAGCTTGTTCGCACCGGAATGG11.9230769230769231No Hit
ATATTGCCGAACAAAGTCTACCACATCAATGCCCCAGCCTTGAAAAAGGT11.9230769230769231No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGAGCCAGCATTTCTTT11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAGC50.0145.03
AGTATTT50.0145.0145
GAGCAAA50.0145.06
GCAAAAA50.0145.08
AGCAAAA50.0145.07
TGAGCAA50.0145.05
ATGAGCA50.0145.04
AACATGA50.0145.01
CAAAAAG50.0145.09
ACATGAG100.072.52
Produced by FastQC (version 0.11.5)