FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.098901098901099No Hit
ACTATCAACTCGAAAAATCTCCTCGTTGACTTACTGATACTAGAAGTATC11.098901098901099No Hit
CCACTTTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCA11.098901098901099No Hit
GCATCAATCCGGGCCCTAGACACCATGGCCTCCCCCATGCTAGATATTAA11.098901098901099No Hit
GACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCGG11.098901098901099No Hit
GAACAACTTCCATTGTTTCAAGGCATGAATTATCAAATATTCCTGGGTGG11.098901098901099No Hit
ACATAGGCTCTAAAGTTTTCAAGGATGGGGAAGTTCGGTGGGAGACTTTG11.098901098901099No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA11.098901098901099No Hit
CTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAG11.098901098901099No Hit
GCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCGACAAGAG11.098901098901099No Hit
GGGAAAGTGGACGACAAACACAGAGACTGTTGCACCCCAGCTCAGCCCGC11.098901098901099No Hit
GTATGGCGTTCTCACTCACTGACCCGCGAGTGGAGCCACACAAATGGGAA11.098901098901099No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT11.098901098901099No Hit
GAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCTCT11.098901098901099No Hit
CTTCTATGGTGGCACAGATCTTCATGATCTCAGAGAACTCTTCGTTCTTG11.098901098901099No Hit
CGATTTGCAGACAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCG11.098901098901099No Hit
CTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTAACAGTCAATGAG11.098901098901099No Hit
GCCGTGGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCAGCTGT11.098901098901099No Hit
CTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCAC11.098901098901099No Hit
GATTTAAGAGTATCAAGTTTCATAAGAGGAAAGAAAGTGATTCCAAGAGG11.098901098901099No Hit
GAATGAAGTGGATGAGCGCAATCAGATACCAAATTACAGCAGACAAGAGA11.098901098901099No Hit
AAGCTGCAGAGAGAGGCAAGTTAAAAAGCAGGGCTATCGCCACACCTGGG11.098901098901099No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.098901098901099No Hit
TGCTAGAGATCTGGGCTCTGGCTCATCCCTGTCATACTGTTTAAGGTCTC11.098901098901099No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTACCAA11.098901098901099No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT11.098901098901099No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCATTCATTACTCATGTCAAAG11.098901098901099No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATAGATGACGAAT11.098901098901099No Hit
GTTTAGTTGAGCCGATTATGGGTGCCATGCTGAGGAGGGGGCTGCGCGGC11.098901098901099No Hit
CCAGTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAAT11.098901098901099No Hit
GGTCTAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAA11.098901098901099No Hit
GTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGT11.098901098901099No Hit
GTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACT11.098901098901099No Hit
AGACAACAAAATAAAATCTGAGAAGACACACATTCACATCTTTTCATTCA11.098901098901099No Hit
GCCCGGAGTCAGTGCTAGTCAAAACTTATCAATGTCTAATCACGAACGGG11.098901098901099No Hit
ATGGCAGACTTTGTGCGACAATGCTTCAATCCAATGATCGGCGAGCTTGC11.098901098901099No Hit
CAATATAAACACTGCCTGTTCCGCCGGCTCCTGGCAGAAACCTTGTTTTA11.098901098901099No Hit
GCCATGAGGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGACTATGTT11.098901098901099No Hit
TAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAG11.098901098901099No Hit
CCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATGGATGCTCTGAAATT11.098901098901099No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC11.098901098901099No Hit
ATAATCATCATCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGAA11.098901098901099No Hit
TGACAGTGATGCGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAAATG11.098901098901099No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTGTGCATTCGGGTCACCAGAT11.098901098901099No Hit
CCCTAAGAAAACAGGAGGACCCATATATAGAAGAGTAGACGGAAAGTGGA11.098901098901099No Hit
GGGCAAGAATACTGACATCAGAGTCACAGCTGGCAATAACAAAAGAGAAG11.098901098901099No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATGTATTCATGCATTTGATTGAATC11.098901098901099No Hit
GGTCCAAGGTCATTGTTTATAATGTTGTTGTTTATACCTGTTACTCGAAT11.098901098901099No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG11.098901098901099No Hit
ACCCATTTGAATGGATGTAAATCCGAATCTACTTTTCCTAAAAATTCCAG11.098901098901099No Hit
GTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAATAT11.098901098901099No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA11.098901098901099No Hit
CACCCATAATGTTGTCAAACAAAATGGCAAGACGAGGGAAAGGGTACATG11.098901098901099No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT11.098901098901099No Hit
TCATCATCCCAGGACTCAGTGATGCTGTGCCATCTAGAAGAAGAGGCCTT11.098901098901099No Hit
AAGAATGTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCC11.098901098901099No Hit
TGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATC11.098901098901099No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11.098901098901099No Hit
GTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGC11.098901098901099No Hit
CGGTCATTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCT11.098901098901099No Hit
AACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCAC11.098901098901099No Hit
CACACTGCCGGTTCCGCCGGCTACTGGGAGCCACCTTGTTTTACGGACCA11.098901098901099No Hit
ATCAGGAACTGGGAATTTGTGAAAATTCAATGGTCACAAGCTCCCACAAT11.098901098901099No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTTGGTTACTTTCCCC11.098901098901099No Hit
CTAATGGATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTT11.098901098901099No Hit
ATTCGACACTAATTGATGGCCATCCGAATTGTTATGGTAGCTGTCTGGCT11.098901098901099No Hit
ATGAAGGCAGTGAACATAAATACGGCCTTGCTCACTGAATCATGTGCAGC11.098901098901099No Hit
GTGCAACACTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAA11.098901098901099No Hit
GCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATCTGAGAACCAATGG11.098901098901099No Hit
ATTTCCAAGTTCCTGATTATACAATGATAATTGTTGACTAACACTGACTC11.098901098901099No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG11.098901098901099No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCTAA11.098901098901099No Hit
ATCAAGATGAAATGGGGAATGGAAATGAGGCGCTGCCTTCTTCAGTCTCT11.098901098901099No Hit
TGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGGTAGCTCCATG11.098901098901099No Hit
AATAAAATCTGAGAAGACACACATTCACATCTTTTAATTCACTGGAGACG11.098901098901099No Hit
ATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCT11.098901098901099No Hit
GTTGACAATAACTTATTAGTCATCAATGATGGGGGAGATCAATGGCCCTG11.098901098901099No Hit
AAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGG11.098901098901099No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG11.098901098901099No Hit
GATTTTGCTCTCATAGTGAATGCACCAAACAATGAGGGAATACAAGCAGG11.098901098901099No Hit
TTCCTGATGTGTACTTTTTGATTATGGCAATATGGTCGACAGTGGTCTTA11.098901098901099No Hit
TCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTC11.098901098901099No Hit
GAGAGGGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGG11.098901098901099No Hit
CAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTACAGTCAATGCC11.098901098901099No Hit
ATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATATGGGGAA11.098901098901099No Hit
GGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGC11.098901098901099No Hit
GTAGAGGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC11.098901098901099No Hit
GGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAA11.098901098901099No Hit
GTCATGTTGTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTC11.098901098901099No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT11.098901098901099No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGG11.098901098901099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGG50.0145.01
GGATGGT50.0145.07
AGAGGGA50.0145.03
ATGGTGA50.0145.09
AGGGATG50.0145.05
GGGATGG50.0145.06
TAGAGGG50.0145.02
GAGGGAT50.0145.04
CATGCTG50.0145.0145
GATGGTG50.0145.08
Produced by FastQC (version 0.11.5)