FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_G09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_G09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775159
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT26340.3398012536782776No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA19870.2563345068560127No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA14920.192476640276382No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC14520.18731640863358356No Hit
TCTCTATATTCCCCCATATCACAAGCCAAGGATGTTACTCCACCTGTCCC12450.1606122098821016No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC11070.14280941071444697No Hit
GTCTCAGACTCTGTGGTTTGAGAAAGGCCAGCCTCTGGTGTGGAAAAGGA10120.13055386056280066No Hit
TGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA9180.11842731620222431No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATACTGGGAAGGGAGACACG8560.11042895715588673No Hit
CCCCTAGTGGTCTCTAACGACTGGACGCAGAGCAGCCGGAGCAGAGCTGG8160.10526872551308829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTG259.382993E-487.00689145
AGCGAAA4250.058.019561
TAGGTAA502.009174E-458.004593145
TACGTGG1353.6379788E-1253.721821
GCGAAAG4600.053.601572
ACTGTCG1101.6752892E-952.724658
GTTCTAA701.6922471E-551.8031851
TCAATAC1501.0913936E-1148.346523
CTGTCGA1203.621608E-948.330929
TCGAGGG1851.8189894E-1243.1198652
GGACAGT3250.042.3961756
CGAAAGC6000.042.3032043
GTCGAGG1903.6379788E-1241.9878431
TTATATG6000.041.094543
GACCGAC1859.640644E-1139.1872377
GTTATAT1303.6871643E-739.051631
ACCGACT1901.2914825E-1038.1559948
TATAGCG1354.971407E-737.590727
GACTCCG2151.2732926E-1137.09596145
GGGTTAT1209.538993E-636.2622261
Produced by FastQC (version 0.11.5)