FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_G02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_G02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855766
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGATGTACTGTGCACGCGGGGAGCGGTGGCGATCAGTTACGGGGAACA18050.21092214460495043No Hit
CTTTATATACTGACAGGCCCTCTGTAGATGTACTGTGCACGCGGGGAGCG14180.1656995019666591No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG12890.15062528775389533No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11890.13893985037965986No Hit
GCTAATAGAGTGAGCTTCACAGGGCATTCCATCCCCTTGTTGAGTCTCAA11560.13508365604616215No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT11520.13461623855119273No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA9800.1145172862675077No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC9540.11147907255020648No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG9460.11054423756026764No Hit
CTGTAGATGTACTGTGCACGCGGGGAGCGGTGGCGATCAGTTACGGGGAA9180.1072723150954817No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA8660.10119588766087925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1550.0102.92091
AGGTACT1200.072.508214
CCTATAC1050.069.059481
GAGCGAA350.003563902862.153531
AGGCCCG502.0089542E-458.0065735
CAATTCG2150.057.3220259
GGCCCGT604.941918E-448.338816
GTATTAA2000.047.133091
TATACTA1255.1895768E-946.4052546
CTCTAAG657.3297863E-444.6230431
CCGTTAA657.338231E-444.6126149
CTATACT700.00105596741.4332664
GGTACTG2300.040.9829035
ATATTCA2200.039.549934
GCACCGT750.001482441438.6710436
CACCGTC1502.6002454E-838.6642657
AAACGGG951.0280809E-438.162212145
CTACACT2500.037.704274
ACCCAAC1209.550988E-636.2562261
CCTTATA1803.1959644E-936.2541052
Produced by FastQC (version 0.11.5)