FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_F12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_F12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281265
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATGTACAGTGCACATGTATCTCTCATAAACTCACTATATAACTCACT20650.7341830657920466No Hit
CATTTACAAACTGTACTTGTGAGAGCCTTCATTGACTGTTAAGTTGTTAC14020.49846230423266313No Hit
GAATTAATACAATCATGTTACATAGAGATGCTATGATGCTAAAAATGGGA13970.49668462126464363No Hit
GTACTTGTGAGAGCCTTCATTGACTGTTAAGTTGTTACACTAATTACATT12300.4373100101327929No Hit
AGTCTATAGTGGCTATATGTAATGGTAGAATGAGGATAGTGAAGTATAGA11780.41882210726539026No Hit
ACTGTAGACTATCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGT11300.40175635077240324No Hit
TTTACAAACTGTACTTGTGAGAGCCTTCATTGACTGTTAAGTTGTTACAC10420.3704691305352603No Hit
CCTCTTAGTTTTACTGTAATGTTGGCTAGGAACTGCTTCCTGCTGGAGCA8410.2990062752208771No Hit
GATCTGGGATACTGAATGTACCACTGTAGACTATCTGCAGAAGAGTAATT8140.28940678719357193No Hit
GCTCTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACT8060.28656249444474075No Hit
GTATGGATTATTCATCACGGTGGCTCCTGTTCTTTACGTCCAGTAGCCCA7730.27482978685581216No Hit
AATTACATTAATGTCTCACTTGTGTGCAAGTTTAGACTGAGAGCATGTGT7570.2691412013581498No Hit
TAGTAGGACAGCACAGAGTAACGTAGGATAGCATAGAGTATAGTAGGATA7280.25883064014363677No Hit
TACAAAGACACACCACAAACATCTCTTACTATGTACAGTGCACATGTATC7250.2577640303628251No Hit
ACATAGAGATGCTATGATGCTAAAAATGGGACAGGAAACTTAAGCACAGT7200.2559863473948056No Hit
ATGTACCACTGTAGACTATCTGCAGAAGAGTAATTACAGGACAAGGTAAC7090.25207544486516276No Hit
CTACTATAGTCTATAGTGGCTATATGTAATGGTAGAATGAGGATAGTGAA6970.24780900574191597No Hit
TTACAGGACAAGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCAGTTCT6940.24674239596110428No Hit
GTTAAGTTGTTACACTAATTACATTAATGTCTCACTTGTGTGCAAGTTTA6600.23465415177857182No Hit
GCATAGAGTATAGTAGGACAGCACAGAGTAACGTAGGATAGCATAGAGTA6580.233943078591364No Hit
ACTCTGATCTGAGCAAAATGATCACTCGAGTCAGTCCTGTAAATCTTTTT6570.2335875419977601No Hit
GTGTGAGACTAAGAGGGGTAGTAACACACACCTCTTAGTTTTACTGTAAT6510.23145432243613673No Hit
CACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTT6490.23074324924892892No Hit
GAATGTACCACTGTAGACTATCTGCAGAAGAGTAATTACAGGACAAGGTA6080.21616624891116917No Hit
CACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTTGCATAA5920.21047766341350682No Hit
GTTACATAGAGATGCTATGATGCTAAAAATGGGACAGGAAACTTAAGCAC5850.20798890725827954No Hit
CTGTTAAGTTGTTACACTAATTACATTAATGTCTCACTTGTGTGCAAGTT5660.2012337119798055No Hit
CATATATACATGCATACATACACACACACACACACACACACACACACACA5620.19981156560538996No Hit
CACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAG5380.19127868735889642No Hit
GTATAGTAGGACAGCACAGAGTAACGTAGGATAGCATAGAGTATAGTAGG5330.18950100439087694No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT5320.18914546779727304No Hit
CTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCAT5250.18665671164204575No Hit
GTGCACATGTATCTCTCATAAACTCACTATATAACTCACTAGGGACACAT5190.18452349208042237No Hit
ATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCT5140.1827458091124029No Hit
TTCATTGACTGTTAAGTTGTTACACTAATTACATTAATGTCTCACTTGTG5120.1820347359251951No Hit
GTATGTAGCTGCCTGATTACTCAAATCCTCTGTGCAACCCCTATCACACA5080.18061258955077952No Hit
CACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAA5030.17883490658276005No Hit
CTACCACACCCAATCAAAAACAAACACAGAAACAAATACATACAAAGACA5020.17847936998915614No Hit
GAGTATAGTAGGACAGCACAGAGTAACGTAGGATAGCATAGAGTATAGTA5020.17847936998915614No Hit
CTATATAACTCACTAGGGACACATGCTCTCAGTCTAAACTTGCACACAAG5010.17812383339555224No Hit
CACACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAA5000.17776829680194833No Hit
CACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACC4980.17705722361474055No Hit
ATCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGTCCTTCCTCTG4980.17705722361474055No Hit
GGATAGCATAGAGTATAGTAGGACAGCACAGAGTAACGTAGGATAGCATA4980.17705722361474055No Hit
CATAGATACTAATTATTCAGTAAAACACCATTCTCTTCTTCAAATCGTTT4970.17670168702113664No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA4930.17527954064672108No Hit
GTGTATTGGTTTCACTGAGTGTGAGACTAAGAGGGGTAGTAACACACACC4920.17492400405311717No Hit
CTGTACTTGTGAGAGCCTTCATTGACTGTTAAGTTGTTACACTAATTACA4890.17385739427230548No Hit
GGGATACTGAATGTACCACTGTAGACTATCTGCAGAAGAGTAATTACAGG4800.17065756492987041No Hit
ACATTAATGTCTCACTTGTGTGCAAGTTTAGACTGAGAGCATGTGTCCCT4790.1703020283362665No Hit
GTACAGTGCACATGTATCTCTCATAAACTCACTATATAACTCACTAGGGA4770.16959095514905873No Hit
CTGTAGACTATCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGTC4760.16923541855545482No Hit
GTTATATAGTGAGTTTATGAGAGATACATGTGCACTGTACATAGTAAGAG4740.168524345368247No Hit
CACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGG4670.16603558921301975No Hit
AGTTTAGACTGAGAGCATGTGTCCCTAGTGAGTTATATAGTGAGTTTATG4650.16532451602581194No Hit
GGTAAGAGATATCATTCATCAGGTACATTCAATTTGTGTTGTTGATGAAC4620.16425790624500028No Hit
GAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTTGCATAAATGC4560.16212468668337687No Hit
CACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTTGCAT4550.161769150089773No Hit
CATACATACATGCAGACATATATACATGCATACATACACACACACACACA4540.1614136134961691No Hit
CACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTTGC4440.15785824756013012No Hit
GTATAGACCATAAAGCACATTTTAGCATGTACGTTATTTTTTACAAGGAG4410.15679163777931845No Hit
ACTATAGTCTATAGTGGCTATATGTAATGGTAGAATGAGGATAGTGAAGT4410.15679163777931845No Hit
AGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCAGTTCTGTCTGGAGTG4390.15608056459211064No Hit
GGCTATATGTAATGGTAGAATGAGGATAGTGAAGTATAGACCATAAAGCA4320.15359180843688336No Hit
CACACACACACACCCCTTCCCATCTACCACACCCAATCAAAAACAAACAC4320.15359180843688336No Hit
GTGATATACTGCTGCTCTATGCTATCCTACTGTACTCTATGCTATCCTGC4310.15323627184327945No Hit
GTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGAGGGGTA4290.15252519865607167No Hit
ATCAAAAACAAACACAGAAACAAATACATACAAAGACACACCACAAACAT4280.1521696620624678No Hit
CTCATAAACTCACTATATAACTCACTAGGGACACATGCTCTCAGTCTAAA4270.1518141254688639No Hit
GTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGAGGGGTAGT4260.15145858887525998No Hit
GTGAAGTATAGACCATAAAGCACATTTTAGCATGTACGTTATTTTTTACA4190.14896983272003272No Hit
CACACACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAG4170.1482587595328249No Hit
CACTAATGGAGGTAAAGACACAGACACAATCAGGCCCTTTGTGGATGGAC4040.14363678381597425No Hit
GTGTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGAGGGG4040.14363678381597425No Hit
GGTACATTCAATTTGTGTTGTTGATGAACATAGATACTAATTATTCAGTA4030.14328124722237037No Hit
GTACAGTAAGCACTCCTTGTAAAAAATAACGTACATGCTAAAATGTGCTT3980.1415035642543509No Hit
CTACAGAGGGAGGGAGAGAAAGAGACAAAGAGAAAGAGAGAGAGTGAGAG3970.141148027660747No Hit
GAACTGGGGAGCTGATCTGGGATACTGAATGTACCACTGTAGACTATCTG3960.1407924910671431No Hit
CCTTTAAACCAGAGAAATGGAAACGATTTGAAGAAGAGAATGGTGTTTTA3930.1397258812863314No Hit
GTGTGTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGAGG3920.1393703446927275No Hit
CTCTTACTATGTACAGTGCACATGTATCTCTCATAAACTCACTATATAAC3890.1383037349119158No Hit
GTAAAACACCATTCTCTTCTTCAAATCGTTTCCATTTCTCTGGTTTAAAG3890.1383037349119158No Hit
GTAGTAACACACACCTCTTAGTTTTACTGTAATGTTGGCTAGGAACTGCT3880.13794819831831193No Hit
GTATATATGTCTGCATGTATGTATGTGTGTGTGTGTGTGTGTATGTATGT3860.13723712513110412No Hit
CACACACACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAAC3800.13510390556948074No Hit
CTCATACACTGAAGCATGGACGTATAAGAAGATGCGTCTGTCACTCAACT3720.13225961282064957No Hit
CAGTATGGATTATTCATCACGGTGGCTCCTGTTCTTTACGTCCAGTAGCC3720.13225961282064957No Hit
ATGTTACATAGAGATGCTATGATGCTAAAAATGGGACAGGAAACTTAAGC3710.13190407622704567No Hit
AAGTAGGAGTGACTCACAGTGAGCTCTATACTGTACTCTAGTAGCCACCT3690.13119300303983786No Hit
GTCTTAATACTACTATAGTCTATAGTGGCTATATGTAATGGTAGAATGAG3660.1301263932590262No Hit
GTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCTCATGT3660.1301263932590262No Hit
AACAAATACATACAAAGACACACCACAAACATCTCTTACTATGTACAGTG3660.1301263932590262No Hit
CCCCTAAGGCTCCCAATCTGCTCCAGCAGGAAGCAGTTCCTAGCCAACAT3640.12941532007181838No Hit
GCTCTATGCTATCCTACTGTACTCTATGCTATCCTGCTGTACTCTATGCT3630.1290597834782145No Hit
GACTAAGAGGGGTAGTAACACACACCTCTTAGTTTTACTGTAATGTTGGC3590.1276376371037989No Hit
TTGTTACACTAATTACATTAATGTCTCACTTGTGTGCAAGTTTAGACTGA3580.127282100510195No Hit
ATGTAATGGTAGAATGAGGATAGTGAAGTATAGACCATAAAGCACATTTT3560.12657102732298722No Hit
GTGTTAGAGAGAGCATGTGTCGGAGATAATTAAAATATGTGCTCGTTAGA3540.12585995413577944No Hit
CACACACCCCTTCCCATCTACCACACCCAATCAAAAACAAACACAGAAAC3470.12337119798055214No Hit
GTTCTAAACTGCACCCTTGTGTCCTTTCAGAGACTACGCCTGTCTGTGGG3440.12230458819974047No Hit
CTCTAGTGCCATCCAAAACACTCATCAACTCTCCTTCTCTGCTCTCAACT3440.12230458819974047No Hit
ATTTAGAAACTGACCAATTCTTTAAAATGTATGTAGCTGCCTGATTACTC3430.12194905160613656No Hit
ATATAACTCACTAGGGACACATGCTCTCAGTCTAAACTTGCACACAAGTG3420.12159351501253267No Hit
GTACATAGTAAGAGATGTTTGTGGTGTGTCTTTGTATGTATTTGTTTCTG3400.12088244182532487No Hit
CACACACACACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTA3370.11981583204451318No Hit
GGATTATTCATCACGGTGGCTCCTGTTCTTTACGTCCAGTAGCCCACAGC3360.11946029545090928No Hit
GTCCTTCACTAGCCGTTACCACCAGACTGGTATTTGCATGGAAGGCTTTG3340.1187492222637015No Hit
CACATACATACATGCAGACATATATACATGCATACATACACACACACACA3330.1183936856700976No Hit
ATGCTATCCTACTGTACTCTATGCTATCCTGCTGTACTCTATGCTATCCT3280.11661600270207811No Hit
GTCTACAAGGCACAGGGCTGCACTCTGCTGTGATATACTGCTGCTCTATG3280.11661600270207811No Hit
GTTGTTACACTAATTACATTAATGTCTCACTTGTGTGCAAGTTTAGACTG3260.11590492951487032No Hit
GTGTTTGGTATGTACTTTTTATACATCCACTGAATTAATACAATCATGTT3260.11590492951487032No Hit
TCTCACTTGTGTGCAAGTTTAGACTGAGAGCATGTGTCCCTAGTGAGTTA3240.11519385632766253No Hit
GTGTATTACCTTTCACCTTCACCTTTGACCTCACACACACACACACATGC3240.11519385632766253No Hit
GTAACATTCAGTCCTTCCTCTGTAAAGACTTCAGTTCTGTCTGGAGTGAT3220.11448278314045474No Hit
GAGTAACGTAGGATAGCATAGAGTATAGTAGGATAGCATAGAGTATAGTA3200.11377170995324694No Hit
GTATTGGTTTCACTGAGTGTGAGACTAAGAGGGGTAGTAACACACACCTC3200.11377170995324694No Hit
GTCAAATCATGCAAACATTTACAAACTGTACTTGTGAGAGCCTTCATTGA3190.11341617335964305No Hit
CAAGTAAACTCATACACTGAAGCATGGACGTATAAGAAGATGCGTCTGTC3170.11270510017243525No Hit
AGTCAGTCCTGTAAATCTTTTTGGAGTATTGGGTGTTGTTTACATTTAGG3140.11163849039162355No Hit
GTAACACACACCTCTTAGTTTTACTGTAATGTTGGCTAGGAACTGCTTCC3130.11128295379801967No Hit
CTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCTCATGTTGGGC3120.11092741720441578No Hit
ATCCTACTGTACTCTATGCTATCCTGCTGTACTCTATGCTATCCTACGTT3090.10986080742360407No Hit
GTGTGTGTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGA3090.10986080742360407No Hit
CAATTAAGTAGCGGAAATTAAGCTCCTTTAATTAAGTCGTCTTTCCCCTA3080.10950527083000018No Hit
CCACAAACATCTCTTACTATGTACAGTGCACATGTATCTCTCATAAACTC3070.10914973423639628No Hit
AGCCCACAGCGTCCCGCATGAGCAGCCTGCATGAAGAGGGGCTCGGCTCC3070.10914973423639628No Hit
GGGCTGCACTCTGCTGTGATATACTGCTGCTCTATGCTATCCTACTGTAC3040.10808312445558459No Hit
GGGTAGTAACACACACCTCTTAGTTTTACTGTAATGTTGGCTAGGAACTG3020.1073720512683768No Hit
CATCTCTTACTATGTACAGTGCACATGTATCTCTCATAAACTCACTATAT2990.10630544148756511No Hit
ATACTGAATGTACCACTGTAGACTATCTGCAGAAGAGTAATTACAGGACA2960.10523883170675341No Hit
ACCACACCCAATCAAAAACAAACACAGAAACAAATACATACAAAGACACA2950.10488329511314952No Hit
GTGAAGAAGATTAAAGTAGGAGTGACTCACAGTGAGCTCTATACTGTACT2930.10417222192594172No Hit
TTTAGAAACTGACCAATTCTTTAAAATGTATGTAGCTGCCTGATTACTCA2930.10417222192594172No Hit
ACATAGTAGCCTGGCAAAATTTTGTCAATACTAACAATGAACAATATTTA2910.10346114873873394No Hit
GTGCAACCCCTATCACACACAGACACAAGTAAACTCATACACTGAAGCAT2910.10346114873873394No Hit
ATATACTGCTGCTCTATGCTATCCTACTGTACTCTATGCTATCCTGCTGT2890.10275007555152614No Hit
ATATTTAGAAACTGACCAATTCTTTAAAATGTATGTAGCTGCCTGATTAC2890.10275007555152614No Hit
ATCACACACAGACACAAGTAAACTCATACACTGAAGCATGGACGTATAAG2860.10168346577071445No Hit
CTTTTTATACATCCACTGAATTAATACAATCATGTTACATAGAGATGCTA2860.10168346577071445No Hit
CACACACACCCCTTCCCATCTACCACACCCAATCAAAAACAAACACAGAA2860.10168346577071445No Hit
GATTAAAGTAGGAGTGACTCACAGTGAGCTCTATACTGTACTCTAGTAGC2840.10097239258350665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.0105.766511
TATGAGG406.1060564E-790.624783
GGTCATT1050.082.8864061
GACGTAC451.2279925E-680.584011
ATATGAG451.2305936E-680.555362
GAGATCC451.2305936E-680.555369
GCGAAAG3200.079.2966842
GTCATTA1200.072.4998252
CTGGGGA1250.069.599834
CGAAAGC3650.069.520383
CAGGGGT754.838512E-967.66654
CGTACAG554.0540835E-665.908933
GCTTCGG350.003563874462.142704145
AGCTGTA350.003563874462.1427047
GGGTGCC606.7930214E-660.416527
GTACAGA606.7930214E-660.416524
TAGAGGC902.0425432E-856.3887524
GAAAGCA4650.054.5697564
TCATTAC1600.054.3748663
CAGATGT400.00604644154.3748662
Produced by FastQC (version 0.11.5)