FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_F06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_F06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697906
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG73761.0568758543414156No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA70121.00471983332999No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA69350.9936868288852654No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA58830.842950196731365No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA57010.8168721862256522No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT47720.6837597040289093No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG46950.6727266995841847No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG40590.5815969485861993No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC37830.5420500755116018No Hit
GCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC35410.5073749186853244No Hit
GAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTACAG33360.4780013354233952No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT31650.4534994684097859No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG31080.44533217940524944No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30610.4385977481208071No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG30610.4385977481208071No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC30510.437164890400713No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT29840.42756474367608244No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT28520.40865102177084023No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC27510.3941791587978897No Hit
GTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAG27160.3891641567775603No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT26490.3795640100529298No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA24860.3562084292153958No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC23290.33371256300991825No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT22670.3248288451453348No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA21740.31150326834845954No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG21440.30720469518817717No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA21020.30118669276378196No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAAGGCAACCAAA20730.29703140537550904No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA20250.2901536883190573No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC20190.2892939736870008No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGAT19980.28628497247480317No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG19440.278547540786295No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG19140.2742489676260127No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC18570.26608167862147625No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA17790.2549053884047422No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA17230.2468813851722152No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG17090.2448753843640834No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA16980.2432992408719799No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA16880.24186638315188577No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG16520.236708095359547No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG16470.23599166649949993No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT16280.23326923683132111No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG16210.23226623642725522No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT16160.2315498075672082No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG15830.2268213770908976No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG15650.22424223319472822No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG15560.2229526612466435No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC14890.213352514522013No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT14710.2107733706258436No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC14530.2081942267296742No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG14030.2010299381292036No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC14020.20088665235719425No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT14000.20060008081317543No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT13940.19974036618111896No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG13690.1961582218808837No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT13510.19357907798471427No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC13510.19357907798471427No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13420.19228950603662956No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG12990.1861282178402249No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA12470.17867735769573553No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA12260.1756683564835379No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT11890.1703667829191897No Hit
CTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCA11880.17022349714718027No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA11660.16707121016297324No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11600.16621149553091677No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC11590.16606820975890738No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC11360.1627726370026909No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA11270.16148306505460622No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA11170.16005020733451208No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT10950.15689792035030506No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC10840.15532177685820153No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA10840.15532177685820153No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACAACAA10820.15503520531418272No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT10810.1548919195421733No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTAAGAGGAATCG10570.15145306101394743No Hit
TGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCT10440.14959034597782508No Hit
GAATAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCAT10210.14629477322160864No Hit
CTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCA9940.14242605737735456No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA9850.14113648542926985No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG9700.1389871988491287No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA9440.13526176877688398No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT9290.13311248219674282No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA9210.13196619602066753No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA9190.13167962447664872No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT8980.12867062326445108No Hit
GCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC8860.12695119400033816No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT8730.12508847896421582No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA8660.12408547856014994No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA8500.12179290620799937No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT8440.12093319157594289No Hit
GTTCATTGATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTC8380.12007347694388644No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA8340.1195003338558488No Hit
CAAGAGAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACT8150.11677790418766996No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC7920.11348233143145352No Hit
GAGAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTAC7770.11133304485131236No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG7740.11090318753528411No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA7700.11033004444724646No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC7670.10990018713121824No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT7670.10990018713121824No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA7590.10875390095514295No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA7460.10689118591902061No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG7420.10631804283098296No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG7410.10617475705897357No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTC7410.10617475705897357No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG7360.1054583281989265No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA7330.10502847088289828No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT7190.10302247007476652No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA7180.1028791843027571No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT7160.10259261275873828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG100.0070898095145.004091
GAGCGAA100.0070898095145.004091
ACGACCA100.0070898095145.004094
GCAGGTA3000.0123.253482
TCTCCGC256.4579854E-6116.01989145
AGTAGAA10200.0115.150311
CAGGTAC3250.0113.772443
AGCGAAA1850.0105.81381
GGTACTG3500.0103.574355
AACTGCG301.597758E-596.68324145
CGAAAGC2100.093.216913
CCGCACG554.1472958E-1092.28856145
CAATCCC950.091.59466145
GCGAAAG2150.091.049082
AATACGC353.4336255E-582.87135145
GCTATAC451.2322871E-680.557833
GTAGAAA15200.077.271922
GGTCAAA9400.076.3585361
ACTGATC4850.076.2337958
AGGTACT5150.073.205954
Produced by FastQC (version 0.11.5)