FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_F05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_F05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCCCGTAACATGGTGGAATAGGAATGGCCCAACAACAAGTACCGT14.0No Hit
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGGGTCTTGTGAATTAGT14.0No Hit
ATCATCGAGTGCGTAAGTCATGACACAAAACTAGAATTTTCCATTTTACA14.0No Hit
GGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAG14.0No Hit
ATCAACAAAGAAGAAAATTGAGAAAATAAGGACTCTTCTAATATATGGAA14.0No Hit
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC14.0No Hit
ACCTAGAGAAACCCAAAAAAATAAAATCTGAGCAGACACAAATTAACATC14.0No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC14.0No Hit
CACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGG14.0No Hit
GAGGCGAAGAGACAATTGAAGAAAAATTTGAGATTACAGTAACTATGCGC14.0No Hit
GAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAA14.0No Hit
CTTTCTCACAACATTTGCCAGTTTGGCCTTCTTTTCATTGACCCCTACTG14.0No Hit
ATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCC14.0No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA14.0No Hit
GAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGG14.0No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCAG14.0No Hit
ACAGTGGATTCGTAAGGACACTGTTCCAGCAAATGCGGGTTCTGCTTGGG14.0No Hit
CAAATAAAATGGTTTCCGATAAGATGTGAACTTGTTCATCGGGGTTTGCA14.0No Hit
GAAGTAATTAAGTCAACACAATAAAGTACATATTAAGAAAAACTCAAGTG14.0No Hit
CGACGCCCCGCCCCCTGCGTTTGCCTCGTGGGCCGCTTAGCCCTGCGCCG14.0No Hit
TTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGC14.0No Hit
GTAGAAACACGGTCGTTTTTAAACAAGTCGCCACTATTTGATGGCCCTCC14.0No Hit
GGAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACTGAGA14.0No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC14.0No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)