FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_E05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_E05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994706
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2850.073.797591
GCGAAAG4800.042.3021242
CGAAAGC6450.033.7292673
ATATACT8600.033.7292674
TATACTT8350.032.1336945
GTTACGC2401.5770638E-930.2158033
CCCGCGT1504.4003158E-528.9984239
TATACTC3301.8189894E-1228.5676675
TATATAC15800.027.9974293
CTATACT3700.027.4392154
CATATAT9050.027.2470441
TTACGCA3306.184564E-1126.3701534
GCGTGCA1100.00965991226.3701532
TACGCAG3151.031367E-925.3237195
TACTTAC9750.025.2806747
ATACTTA10700.025.0762926
GTATATA13100.024.3596151
CTTACAC10000.023.9236989
CCTATAC3152.8574505E-823.0215633
TATACAG8600.022.7672565
Produced by FastQC (version 0.11.5)