FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_E02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_E02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951321
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA110621.162804142870808No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG91270.9594027673098776No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA84920.8926534786891072No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC79430.8349442512043779No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT79050.8309498055861271No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA74000.7778657256593726No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG62800.6601346969109271No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG56610.59506728012942No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG51820.5447162419414687No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50820.5342045429460718No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC48400.5087662313772113No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT47110.4952061396731492No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT46890.4928935658941619No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG43720.4595714800787537No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC43440.4566282043600425No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG42330.44496021847515194No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC42310.444749984495244No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40470.4254084583437136No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG39020.4101664948003881No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT37110.39008914971917996No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT36110.379577450723783No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG35120.36917086871834004No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA34500.362653615341194No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC33320.35024981052662557No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG31950.3358487829029318No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC29860.31387933200255225No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA29830.31356398103269034No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG29300.30799278056512996No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT29130.3062057917359125No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG27440.2884410204336917No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT27010.283920989865671No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG26840.28213400103645353No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG26720.2808725971570059No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC26330.2767730345488011No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA25850.27172741903101055No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA25770.2708864831113788No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT24290.2553291685981914No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC24140.2537524137488818No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC23320.24513282057265634No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA23310.24502770358270234No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA23240.24429188465302457No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG22050.23178296284850222No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT22040.23167784585854828No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG21350.22442477355172438No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA20960.2203252109435196No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20790.2185382221143021No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA20630.2168563502750386No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC19790.20802652311890518No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC19400.2039269605107004No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA19130.20108880178194322No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA19090.20066833382212734No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT18850.19814552606323207No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC18830.19793529208332414No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA18790.19751482412350826No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT18270.19204874064590186No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA18010.18931569890709865No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG18000.18921058191714468No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA17960.1887901139573288No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG17610.1851110193089399No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA17550.18448031736921605No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG17510.1840598494094002No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT17310.1819575096103208No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG16990.1785937659317938No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC16110.1693434708158445No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG15970.16787183295648894No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC15960.16776671596653497No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA15500.16293133442865237No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA15470.16261598345879047No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA15440.16230063248892856No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA15310.16093411161952695No Hit
TCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCA15200.1597778247300333No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC15160.15935735677021742No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA14960.15725501697113803No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA14860.15620384707159832No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA14580.15326057135288718No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA14540.15284010339307133No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT14460.15199916747343956No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT14210.14937124272459032No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG14090.1481098388451427No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA13930.14642796700587918No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC13770.14474609516661568No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC13740.14443074419675378No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC13480.14169770245795058No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT13420.14106700051822677No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG13380.1406465325584109No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA13230.13906977770910134No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAA13160.13833395877942356No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG12770.13423439617121874No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA12650.13297299229177112No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC12580.13223717336209334No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC12330.1296092486132441No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA12270.1289785466735203No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG12240.1286631957036584No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC12100.12719155784430283No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA12060.12677108988448693No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT12050.12666597289453296No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT11940.1255096860050393No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAAGGAATGAACAAGGACAAACC11540.12130500640688054No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT11470.12056918747720274No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC11460.12046407048724878No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG11360.11941290058770909No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG11290.11867708165803131No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT11250.11825661369821543No Hit
AAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAG11200.11773102874844557No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG11140.11710032680872176No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT10900.1145775190498265No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA10870.1142621680799646No Hit
GAATGAAGTGGATGATGGCAATGAGATGCATTAAGCATCAATGAACTGAG10760.11310588119047094No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA10650.11194959430097728No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC10610.1115291263411614No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA10530.11068819042152965No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC10490.11026772246171376No Hit
GGAGCAAAACAAACGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTA10340.10869096761240422No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG10340.10869096761240422No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT10270.10795514868272645No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT10260.10785003169277248No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT10230.10753468072291056No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA10070.10585280888364705No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT10000.10511698995396927No Hit
TCTCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC9910.10417093704438354No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA9850.10354023510465973No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG9770.10269929918502799No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT9670.10164812928548828No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG9590.10080719336585653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG100.0070900237145.00537145
TTGCCGG100.0070900237145.00537145
AGTAGAA15350.0114.776231
GCAGGTA11250.0111.4930042
CAGGTAC12200.0102.811173
GGTACTG12550.099.943935
AGCGAAA2500.092.803431
GCTATAC259.385227E-487.003213
GTATAGC259.385227E-487.003211
GCGGACC259.385227E-487.003214
GGTCAAA8750.082.03161
CCCTTAC554.3930413E-879.0938341
ACTGATC16350.078.0415958
GTACTGA16550.077.102556
AGCAGGC4100.074.271043
ACTGCGG300.001935429672.502681
AGGTACT17950.072.300734
TACTGAT17750.071.886217
GTCAAAT10000.071.777662
GTAGAAA24700.071.6220862
Produced by FastQC (version 0.11.5)