FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAGAAGATAATTTATATTATAGGTAAAATTTCACATAACCCCATCAC11.7543859649122806No Hit
CCCCTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA11.7543859649122806No Hit
CCTCTGACTGCTTTTATCATACAATCCTCGTGTGAAAATACCAGCGCCAC11.7543859649122806No Hit
ACGGAAATGATTCCTGAGAGAAATGAGCAAGGACAAACTTTATGGAGTAA11.7543859649122806No Hit
GGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTC11.7543859649122806No Hit
CGGGCTGGAGGTCAGTAAGTATGATAGCGGCCAGGTTCCGGTGCATGAAA11.7543859649122806No Hit
GGCCTGAGAGCCTGACAATAACTTACTCATCTTCATTGATGTGGGTGAGT11.7543859649122806No Hit
GTATCAGCAGATCCACTAGCATCTTTATTGGAGATGTGCCACAGCACACA11.7543859649122806No Hit
GTTAGTATCCTGGCTCCCACTTCGTTAGGGAAAACAACGTCCATGATTAC11.7543859649122806No Hit
GGCTCGCACTAAGTAAAACTCTATGTGACAGCTGTCAGTATCCATAGAAC11.7543859649122806No Hit
AACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGC11.7543859649122806No Hit
CTGTTAAAATTCAGTGGTCCCAGAACCCTACAATGCTATACAATAAAATG11.7543859649122806No Hit
GTAGTACATTTCCTCGGTGGTCCCGTATTCTAAACAAAAGGGCAATGCTA11.7543859649122806No Hit
GCAATCGACTGTTCGTCTCTCCCACTCACTATCCGCTGAATCAATCTCCT11.7543859649122806No Hit
GTATCAGCAGATCCACTAGCATCTTTATTGGAGATGTTCCACAGAACCCA11.7543859649122806No Hit
GTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTTGACATAAATCC11.7543859649122806No Hit
GGATAATAAGTAAAAGGGAAATTCTTAAAATTCAATCGTCACAAGACCAA11.7543859649122806No Hit
TAAACGAACTAAGAAATCTAATGTAGCATTCTCGCACACGCGAGATACTC11.7543859649122806No Hit
GTCATGCAGATATCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTT11.7543859649122806No Hit
CTTGCCCAAGATCTCATGAAGCACATCAAATGGGGGAAGGGAAGAGTTGA11.7543859649122806No Hit
TATGTCAACTCTCCGACGTATTTTGACTTGGTTTCTAAAATGGACAGGGC11.7543859649122806No Hit
GGCTGTGACATGGTGGAATAGGAATGGACCAATAACAAATACAGTTCATT11.7543859649122806No Hit
CTGGAGCTCTGCTAGCACCTGCTTCCTAGCCATGAGGTAATTGGGATTTA11.7543859649122806No Hit
GCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTT11.7543859649122806No Hit
GAATGAGGAATCCCCTCAGAACAGCGGCCTCCACTCCAGCTGTGCCTTCA11.7543859649122806No Hit
TGATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAG11.7543859649122806No Hit
CAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGACTT11.7543859649122806No Hit
AAACAAGGTCGTTTTTAAACTATTAGACACTAATTGATGGCCCTGCGAAT11.7543859649122806No Hit
ATGTGATGGGAATGATTGGGATATTGCCAGACATGACTCCACGCATCGAG11.7543859649122806No Hit
GTGGAGTATACATCTGTTCCCAGGATGTTCCTTGAGTCAAATGCAACACT11.7543859649122806No Hit
CATCAATACAATTCTCAGTCAAAAAGAGATTGATTATTTAAAAAAAAATT11.7543859649122806No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCACTAA11.7543859649122806No Hit
TCAGTGCTCTTATTAGATAGCCTCTCTTATTCTGTCTTTGGTTTTTCTTC11.7543859649122806No Hit
CCGTGGATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTAATCCTTC11.7543859649122806No Hit
GTGCTGACAAACCCACTGTATTGGCCTCTAATGGCATTAGGTACTAAAGA11.7543859649122806No Hit
CTCATTTACTGTGAATGTGAGGGGATCAGGAATGAGAATACTGGTAAGGG11.7543859649122806No Hit
GGGATATGAAGAGTTCACAATGGTGTTGAGAAGAGCAACATCAATACTCA11.7543859649122806No Hit
TTACCAAGAATACCTCCACTTCCACAATTAGCGTACACTCCTTTTTCAAG11.7543859649122806No Hit
CCCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT11.7543859649122806No Hit
CAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTACAAAC11.7543859649122806No Hit
TCCCAGAAGCCTACAATGCGATACAAAAAAATGGGATTAGAACCCTTTCC11.7543859649122806No Hit
GCATGGGGACTGAGAAGTCGCGCCTCCTTGAGTGTGGAAGGCTTCACAGT11.7543859649122806No Hit
ATGTGATGGGAATGATTGGGATATTGCCAGACATGCCTCCAAGCAGCGAG11.7543859649122806No Hit
CTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACTGA11.7543859649122806No Hit
ATTGTGAACTCTTCATATCCCTCATGCACTCTTATCTAAAATGAATGAAG11.7543859649122806No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTAAAAAAACGGTCAATGCTC11.7543859649122806No Hit
GGTAAACAAAGTGAATATTACGGTTGCAATTATTTAAGGGAACTTAGACA11.7543859649122806No Hit
GTCAATTATTCATACCATTCATGTACTATTATTTTCAGAATTTGTAGCTT11.7543859649122806No Hit
TCTCTGTTCCCTGTGTTTCACTGACATCCTCGGGAGACACAAGTACAATT11.7543859649122806No Hit
ATGCAGGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGG11.7543859649122806No Hit
GGAACATGCTGGGAACAGATGTATACTCCAGGAGGGGAAGTGAGGAATGA11.7543859649122806No Hit
TACGATGCAATGGACGTGGTTTGGTTGCCTTGGACTAAGTGACTGCACGA11.7543859649122806No Hit
GACGCTGAGTAATTTATTGTCAGTTTCTCTGTTCCCTTTGTTTCACTGCC11.7543859649122806No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT11.7543859649122806No Hit
ATATGCAAGGCTGCAATGGGACTGAGAATTAGCTAATCCTTCAGTTTTGG11.7543859649122806No Hit
TCCAATTACATCAGCAAAGAGGATAAAGGAAATGATTAATGATAGAAATT11.7543859649122806No Hit
GGATTAGAGTGCGGAGTGGGGGTGGAGATGCTGCCGCGCGGCGGGCGAGC11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCT50.0145.09
CAACACA50.0145.0145
TCAATAC50.0145.03
CATCAAT50.0145.01
CAATACA50.0145.04
TACAATT50.0145.07
AATACAA50.0145.05
ATCAATA50.0145.02
ATACAAT50.0145.06
ACAATTC50.0145.08
Produced by FastQC (version 0.11.5)