FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_D11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_D11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840243
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1950.055.7751161
CGAAAGC2100.055.2406353
GCGAAAG2500.046.4021342
GTAGAAA6950.044.8608471
CCTTATA4250.040.9430582
TATACGT1001.3908834E-436.2516674
CAATTCG2700.034.9069379
CCGGATT850.002741119134.119217145
GTCCATA1102.434312E-432.958021
AGTAGAA6200.032.7453881
TAGACAG6000.030.209725
GTATGGC1306.482593E-427.884247
CCTTAGA6250.027.841282
TAGAAAC10800.027.5244142
CTTAGAC5800.027.5012653
TTAGACA5400.026.8530864
GTATAAA2456.429218E-826.6354621
CTTAACG2207.476683E-726.3664171
TTATACT6050.026.364854
GTATTGT1958.745146E-626.0283871
Produced by FastQC (version 0.11.5)