FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_D10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_D10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597466
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA20960.3508149417707451No Hit
GTCTAATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGG12570.21038854093789439No Hit
GTCCTGAATGCCTCCTCCCATGGCTTGGTCCTCCACAGCTTGGAGCCTGC9860.16503031134826082No Hit
GTACAAGCCTTACCCTGATGACGTTGGCCCTAGAGGAATCTGAGCTGCAT9460.15833536971141454No Hit
CCTTAGGGAAGGGATTGGCCACCGGCATCCGGGCATCCCGCAGCTCTTCG9030.1511383074518048No Hit
GTACAGGCTGGGGGCGAAGGCTCTGCTTTCACACCCGGGGGCGTTCCTCA8940.1496319455835144No Hit
AAATAGAAGTGAGAAAAGAAAAAGGAAGTAAAATACAAATATCTGGGCCT8200.13724630355534875No Hit
GTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACGGGGAGGCT8010.1340662062778468No Hit
TATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCACA7610.1273712646410005No Hit
CTTCTGGACCAATGCTCAAGACCAGTCCTTCTCCACTGTCCTGAATGCCT7030.11766359926757339No Hit
CCCTAAGAGGGGCTGAGTAGAGTCACAGCGGAGGTAATGCCCTGGACCTG6810.11398138136730794No Hit
CCCTGGGCCCTGGCTTCTTCTCTTCTTCTTCTAGCACCGTGATCTTGGTG6240.10444108953480198No Hit
CATAGTAGGTATGCCGGTTGCTGCAGCCCACGTCCGAATAGCGGCGGGAG6220.10410634245295966No Hit
TTCTAGCACCGTGATCTTGGTGATGGGTGTCATGCCGGGGTCCCGAATGC6180.10343684828927505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC203.8647986E-4108.75345145
GCGAAAG1750.091.161012
AGCGAAA1800.084.6001741
CGAAAGC2150.074.194613
ACGGGTG451.1927849E-464.451885
TATTACG350.003562989462.155232
GGTCATA350.003564171362.150036
GACCCGT450.00963285848.3389136
GGTCGGT1453.8380676E-1044.997667
CAAACCT2550.042.6448448
TTCAACG1201.9724757E-742.289477
CAACGTA1053.822892E-641.4264229
ATAGACC3550.040.849793
TATAGAC4000.038.070082
AAGGTCG1752.4228939E-937.290025
TCTGCCG800.002035642736.251152145
CGGGGTA800.002036479336.248129
TCAGACG1458.682782E-735.0040444
CAGACGG1751.0070107E-733.1466835
GTATAAG1102.4321362E-432.961111
Produced by FastQC (version 0.11.5)