FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_D07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_D07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA60.2932551319648094No Hit
TTCTAGCACCGTGATCTTGGTGATGGGTGTCATGCCGGGGTCCCGAATGC50.24437927663734116No Hit
GGATACGGGAGGGAGATGTGGATAGAATGCTTAACAGGAGCTGGAAACCC50.24437927663734116No Hit
GGGTAAAGCAGAGGGCTTAATTAGGAGATTAACCAGGAGGAAGACTAGGT50.24437927663734116No Hit
GTATGGGGCACTCCGGGCCGAGGGGGCAGCAGGCTTGGGGCCTTCGGCCA50.24437927663734116No Hit
TACTAACAGAGTGTATAAACCTAAAGTTGTTGAAGCTTTACAAGGAATGT40.19550342130987292No Hit
GCCCTCATGTCTGGCTGACCAAGCACAAATGTGGCTACGTACGCCCGAGT40.19550342130987292No Hit
CCTGGGTGCCGACCATCATGGTGGAGGAGACCTTCCAGCTGCCCAGCATT40.19550342130987292No Hit
GCCATGATGTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACG40.19550342130987292No Hit
CCCTTTATTCTTCTCTTTCTTTTCCTGGAACACAGCAAGTCCCAAGATCA40.19550342130987292No Hit
ATACTGCACTTTTGTTATTTCTACGGATGGCTCTGTTAGAGCTTGTGGTA40.19550342130987292No Hit
GTTCCTGCATGCTGTAGTAGGTGGTGTCCGCACATCTGGCTGACTTTCAC40.19550342130987292No Hit
TATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCACA40.19550342130987292No Hit
GTCTAATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGG40.19550342130987292No Hit
GTACAGGCTGGGGGCGAAGGCTCTGCTTTCACACCCGGGGGCGTTCCTCA40.19550342130987292No Hit
GGCCTGGACCAGGGTGGGGAGGCCACAGGTCCCTGAGCTGTCATTGGACT40.19550342130987292No Hit
GAGCACGATAGCCGTGGCATACAGAAGGACAGAAAGCAGCGCCAGTCCTG30.1466275659824047No Hit
ACATTTAACTGACTGAGCCACCCTGGCATCCCTATAAACAAGTTTTTTTA30.1466275659824047No Hit
CTTCTTGTGCAGTGGCAGAGACTCCAACTTATCTACAAGACAGCGGAGGC30.1466275659824047No Hit
CTATTGAAATTCCTCTTTGCAAGTCAAGAGGAATCTACTTAAGATGATCT30.1466275659824047No Hit
CCATGATGGTCGGCACCCAGGAGCCCAGGCTGCTGTGGGCCGGGTCTGGG30.1466275659824047No Hit
AATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGGCTTA30.1466275659824047No Hit
GTCATTGGACTCCCTGGGCCCTGGCTTCTTCTCTTCTTCTTCTAGCACCG30.1466275659824047No Hit
CCCGAATGCTGGGCAGCTGGAAGGTCTCCTCCACCATGATGGTCGGCACC30.1466275659824047No Hit
GGCTTAAACATGGGATCATAGGTCTAAGCGGGCACATGTTGTGTGGCTGA30.1466275659824047No Hit
TCTTTAGACAAAGCAATAGTATGTGAACTGCCACAAGAAACCTCTCCAAC30.1466275659824047No Hit
ATGAGAAAGAGCCAAACAATGACTGTCTCCAATAGAAATGTCAACTATTC30.1466275659824047No Hit
CTGGTGGACAGAGGACCTTCCGAGAGACTCGGGCGTACGTAGCCACATTT30.1466275659824047No Hit
GTGTCCCAGCTTGTTAGATTTCTAGCATAAAAGCCTTGTGACTGCAAGAA30.1466275659824047No Hit
GTTCAGAAACTCTTCACGGGGAGGCTTGAGGGCAGGCACCATCTCTGCTT30.1466275659824047No Hit
CCTCATGGCTACCAACAAGGCCGGGGCCGCCAACAGTACAAGCCTTACCC30.1466275659824047No Hit
CCCCAGAGCTGCATCTAAAGCACATTGGCAAGACCTGGGCCCAGCTGGAG30.1466275659824047No Hit
CTCTGCTTCACTGCAGGAGAAAACAGAGAGGAGACACGGGCCTCCGAGTG30.1466275659824047No Hit
AAATAGAAGTGAGAAAAGAAAAAGGAAGTAAAATACAAATATCTGGGCCT30.1466275659824047No Hit
GCTTTCTGTCCTTCTGTATGCCACGGCTATCGTGCTCTGGCCACTCTACC30.1466275659824047No Hit
CATCAAAGGACTCCAGCCTCCACTGCAAATTCCAGGGGGAATTAAGCTTC30.1466275659824047No Hit
ATTTCCATGGGTGTAAAGTGAAGACAGTAATGCCTTTCTTCCCTTGCAGG30.1466275659824047No Hit
GAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGGCACAAACAAACG30.1466275659824047No Hit
GCCTTACCCTGATGACGTTGGCCCTAGAGGAATCTGAGCTGCATGTCCTC30.1466275659824047No Hit
CCCTGGGCCCTGGCTTCTTCTCTTCTTCTTCTAGCACCGTGATCTTGGTG30.1466275659824047No Hit
GAAAAAGGAAGTAAAATACAAATATCTGGGCCTTGATGCAAAGACAAAGA30.1466275659824047No Hit
CTACAAGACAGCGGAGGCCTTCAAGCTCAAAATGGGTCAGATGCACCTGC30.1466275659824047No Hit
GTCCTGAATGCCTCCTCCCATGGCTTGGTCCTCCACAGCTTGGAGCCTGC30.1466275659824047No Hit
GGATAAGAGGCCGGCGGGGTGGACAGGCCGCTGTACAGGCTGGGGGCGAA30.1466275659824047No Hit
CCGTAGTGCATGTCACTGCGTGTCTCCTCTGGGGCCGCCTCCTTGGGGGA30.1466275659824047No Hit
GTACAGTTCAGTATAATCAGTGTTAAAACTCTTACATAATTCAGAACTTC30.1466275659824047No Hit
GTGTATTTCAACGTAAAATATTTTCCTGATACATTTTATTCTTCCTCTTC30.1466275659824047No Hit
GTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACGGGGAGGCT30.1466275659824047No Hit
GAGTGAGGGAGATGCGTGGGTTGGGTAACAATGTTCAGGGAAGACCTCTA30.1466275659824047No Hit
ATCTTATACCATGAGCATTTTCCCACAATCATGTGCTATCCCTCCCTTAC30.1466275659824047No Hit
GTCCACCAGTTCTCACTCGGAGGCCCGTGTCTCCTCTCTGTTTTCTCCTG30.1466275659824047No Hit
CATAGTAGGTATGCCGGTTGCTGCAGCCCACGTCCGAATAGCGGCGGGAG30.1466275659824047No Hit
TCAATAAGCTTGGGTCTCAAAGCAGTAGCTTCTGAAGAACCACAACCTAG30.1466275659824047No Hit
ACACCATCTTCTGGACCAATGCTCAAGACCAGTCCTTCTCCACTGTCCTG30.1466275659824047No Hit
GGTGAGGGCAGGGAGATAGAAGATCTAGATCTTCCACAGGGAAAAGGTAT30.1466275659824047No Hit
ATACAAATATCTGGGCCTTGATGCAAAGACAAAGAACTGCTTTGTTTCTC30.1466275659824047No Hit
GTCTAAGCGGGCACATGTTGTGTGGCTGACTCGCTGCTCTTGTCTGGGCC30.1466275659824047No Hit
GTGCTAGAAGAAGAAGAGAAGAAGCCAGGGCCCAGGGAGTCCAATGACAG30.1466275659824047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGCT100.006573973145.04
GCCTGGG100.006573973145.02
GGGCTCC100.006573973145.06
CCCCCCC400.007394976418.12585-89
Produced by FastQC (version 0.11.5)