FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_D06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_D06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000865
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12090.12079551188222187No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC11230.11220294445304811No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10820.10810648788797689No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10030.10021331548210796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA2900.052.4980287
AGCGAAA1700.051.182221
CAATTCG2450.050.3042349
CCTTATA3800.040.0662882
ATATTCA4500.038.667154
TTATACT4350.038.3338134
GTAGAAA7150.037.5216981
CGAAAGC3000.033.8337552
AGTAGAA5500.032.958251
CAGGTAC4000.032.6254043
GGTACTG4000.032.6254045
ACGACCA900.00362801232.2226225
GCGAAAG3200.031.7223151
CCATGTC8100.030.4309569
TTTAAAC4600.029.9460243
GGATAGC4600.029.9460245
GATAGCA4850.028.4024146
GCAGGTA4900.028.1125952
GATAGAC2853.237801E-1027.9828032
TTATAGG2903.947207E-1027.503091
Produced by FastQC (version 0.11.5)