FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_D04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_D04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857458
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1800.064.457591
CAATTCG2800.054.3734059
GCGAAAG2600.047.4135132
CGAAAGC2500.046.4094623
GGTTCGC951.917866E-645.7988132
GGTTATA803.729711E-545.321741
AGTAGAA6700.042.21011
GTAGAAA6850.041.285792
TTATAGG3900.040.9057771
CCTACCG1354.9623486E-737.6002585
CCTTATA4200.036.2573932
TCGGAAC800.002036747336.2489368
TACCGCC1551.4650213E-632.7409747
GCTTATA1352.1388634E-532.228791
GTCTAAG5200.032.073851
CTACCGC1601.8737192E-631.7252186
TTCGCAG1402.7423985E-531.0777664
TGTATAC1753.767802E-629.0059152
TTATACT5350.028.4637474
ACAATTC5350.028.4571088
Produced by FastQC (version 0.11.5)