FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCAT12.380952380952381No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA12.380952380952381No Hit
ATTGAATACTGGGGAATTGCCTCTTAACAGTATCCACAACCATGATCATC12.380952380952381No Hit
GCCGGTACTGATCCAAAATGGAAGCCTTTGTGCGCCAATTCGTCAATCCA12.380952380952381No Hit
TATCTCTTGTATTCTTTGCAAAAAATGATAAATCCGATCATGAAAGAAGA12.380952380952381No Hit
GCACATTCGACACAATGGAACCCTTAAACATAAACCAACCATAATAATAA12.380952380952381No Hit
AATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAA12.380952380952381No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG12.380952380952381No Hit
ATTCCAGAGAGGAATGAACAAGGCCAAACCATCGGGAGAAAAACAAACGA12.380952380952381No Hit
AGCAAAAAGAAGTACTATATAAATAAGACAGGGACATTTGAATTCACAAG12.380952380952381No Hit
GTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACA12.380952380952381No Hit
CTCGGTGCTGGATTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTC12.380952380952381No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT12.380952380952381No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG12.380952380952381No Hit
GGATCAGACCGAGTGATGGTATCACCACTGGCCGTAACATGGTGGAATAG12.380952380952381No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGGACAAAGAGGAA12.380952380952381No Hit
GTGGATTACTGATCGTCTTTTTTTCAAATGTATTTATAGTCGCTTTAAAT12.380952380952381No Hit
GCTCTCTTCGTCAAGGGTGTAGTCCGCTTTGGTGGCCATCTAATCTCCAC12.380952380952381No Hit
ATCATCATCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGAAGAT12.380952380952381No Hit
GGTCAAATATATTCAATATGGACAGAATAAAGGAAGTGAGAGATCTAATG12.380952380952381No Hit
CCTATATGTGTATCTGTAGTCGTTGATGAACAATTGAAGAGCCATCTGGG12.380952380952381No Hit
CTTCATCAAGGGTGTAGTCCGCTTTGGTGGCCATCTCCTCTCCAGTGAAT12.380952380952381No Hit
ATGGAAGACTTTGTGCGACACTGCTTCAATCCAATGATCGTCGAGCTTGC12.380952380952381No Hit
TCAATATACTGGTGGGATGGGCTCCCATCATCCGACGATTTTGCTCTCAT12.380952380952381No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACAGG12.380952380952381No Hit
TCTTTTCATAGCCCCCTACTGGGAGCCCAGAGACTTCAAGCTTTTCGAAA12.380952380952381No Hit
AATGGATACTGATTATCGGGGAAGAATTTGTAATAGACTGAATCACTTTG12.380952380952381No Hit
CCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCC12.380952380952381No Hit
ATTTTGGACAGTATGGATAGCAAATAGTAGCATTGCAACAACTACTTCAG12.380952380952381No Hit
GTTGTAAGGCTTGCATAAATGTTATGTGTTCGAAAATAGTATCTGTCTCT12.380952380952381No Hit
ATAAAAGAATAATCAATAAAATCACAAAAATATTAATTGTGAATAATTTG12.380952380952381No Hit
ATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA12.380952380952381No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCATGTTCCAGGTTGTCGCT12.380952380952381No Hit
ATTAAAGGTGACAGGACTCAGGGAAAGGGAATGTTTTTGAAAGATTATAC12.380952380952381No Hit
TCTTCTTGGTCAAGTTGTCTCTTACTCTCCTTTTTATTTGAAAGTGGGGT12.380952380952381No Hit
AAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATCGATT12.380952380952381No Hit
CAATGCTGTGGTAATGCCAGCCAATGGTCCAGCAAAAAGCATGGAATATG12.380952380952381No Hit
TCATTGCATGTCCCATCTGCCTAGTCTGAGTAGCAACCTCCATGGCCTCC12.380952380952381No Hit
GTGCCATCTATTAGAAGAGGCCTTATTTTATCAATTTTCTTCTTTGTTGA12.380952380952381No Hit
CTGCTTTTGATGAGAGAAGAAATAAATACCAATAAGAGCATCCCAGTGCT12.380952380952381No Hit
GTGTATGAAGAATTCAAAATGTGTTTGAGAAGATCAACATCTAGTAGAAG12.380952380952381No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)