FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_C11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_C11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321354
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGACCAATCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACA10150.31585105522258944No Hit
GTACAGAGAATTACTTAATATGTTAGTAGAGTGGATATTAGATGGTGGAG8760.2725965757389048No Hit
TGTTAGTAGAGTGGATATTAGATGGTGGAGACAATTAGTCGACCGCTATG8590.26730645954305843No Hit
CTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAAGCT8520.2651281764035923No Hit
GCTATTAAGGGTAGGCAGGAGAAGATGCATTTAAGACTTACTTAGGAGGT8150.2536143940949856No Hit
ATATTTATCTATGGCTTCATTTTAGTGTCAAAACTCACACACGCGAGGAA7780.2421006117863789No Hit
TCTTTATGCTCCCCAGAAAACCATTTACTAATGCCCATGCTTACCTCCTA7380.22965327956085813No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT7320.22778617972703No Hit
TCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAGTC7190.22374079675373573No Hit
GCATAAAGAAGGATATCAAAATAGGGGTGGCGAAGGAACTCGTGTTGCAA6910.21502766419587124No Hit
GTACTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAA6830.21253819775076707No Hit
AAATTAGAGGATGTAAATTGATATTTATCTATGGCTTCATTTTAGTGTCA6560.20413624849854056No Hit
ATCTATGGCTTCATTTTAGTGTCAAAACTCACACACGCGAGGAAGCGTGT6540.20351388188726452No Hit
CCCCAACCCCACGCACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGA6490.2019579653590744No Hit
GAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAGTCATA6330.19697903246886614No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTG6290.19573429924631403No Hit
CCCTGAAAGTGTGCCTTCTCCTCTGGGCCTCATCACAGTTCACGGTGCCA6090.18951063313355365No Hit
AATCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAG6040.18795471660536356No Hit
CCTCTGGGCCTCATCACAGTTCACGGTGCCAGTACTCTCAGGTGACTGGT5650.17581856768548082No Hit
GCTTTGGTTCCCGCCCACAGTGTCAAAAGTCATAACTCGAGCGAGCAGAT5260.16368241876559805No Hit
GTGCCAGTACTCTCAGGTGACTGGTCGGAAATCAGGAGTCCCCCTCAGAC5240.16306005215432204No Hit
CTATGAGCCCAGGCGGTGAGTACTATTATTGTTCCCATTTTACAGTTGAG5140.15994821909794185No Hit
ACTCTACTCTTCCCTGCCTCCCACTCCATTGCTTTGGTGTAGTTCACAAA4980.15496928620773354No Hit
CTCCAGAGGCGCCCTACTCACTCGCTTTCAGGTTTGTCCAGCTAGCCAAA4950.15403573629081946No Hit
ATATTAGATGGTGGAGACAATTAGTCGACCGCTATGGTAATCTGGCTAAA4830.15030153662316323No Hit
CACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGAAAGTGTGCCTTCT4810.1496791700118872No Hit
GTTATATCCCTGCATCACGGCCATTCAATAGGCCCTGCTCAGGTCATTCT4690.14594497034423096No Hit
GTCTAAAACCTGGTGCTAGGTCACAGAGCTTGTCACAAGAGAGGCAGGAT4570.14221077067657475No Hit
GTGTCAAAAGTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGT4530.14096603745402267No Hit
GGTTAGGACATCTGGTCTGTGGGTCTGGACTCCGAGTGCCAGGGTCTGAG4500.14003248753710862No Hit
GTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGTATGATAAAT4490.1397213042314706No Hit
CCGTAGAAGCTCCTGAAGAATGTCTTTCAGTCTCTGTCTTACTGGACCAC4470.13909893762019454No Hit
GGCATGGAGTGTGGAGAACACGCAAGCATACTGGGACGATTGAGTGTTGA4370.13598710456381435No Hit
GACATAATGTGAGTAAAGCCCTCATCACCTGCATGGCTGCTATGATAAGC4350.13536473795253834No Hit
CTACTAACATATTAAGTAATTCTCTGTACCGTGACTTCCAGATAGAACAA4340.1350535546469003No Hit
GTCCAGGAGGTCTGTGCTCCAGGAGGTCTGTGCTCCATCTTCACGGAGGA4330.13474237134126227No Hit
CCCTCAGACAGAGGTGGAGGTGGCAAGCACGCGGCACCTACTCTACTCTT4330.13474237134126227No Hit
CTTAATAGCTGTGCCCGAAATATGAAACTCTTGTTTTATTTTAGCCAGAT4280.1331864548130722No Hit
ATGGTAATCTGGCTAAAATAAAACAAGAGTTTCATATTTCGGGCACAGCT4230.13163053828488208No Hit
CCCCTATTTTGATATCCTTCTTTATGCTCCCCAGAAAACCATTTACTAAT4090.12727397200594984No Hit
GCTTTGGTGTAGTTCACAAATCGTTTATGGTCCTTTTTCCCACTGAACTC4000.12447332225520766No Hit
ATTGAGTGTTGACCGCGAGACAGAATGTCTGCAGAAGGTGGGAAGATTGC4000.12447332225520766No Hit
GAATTACTTAATATGTTAGTAGAGTGGATATTAGATGGTGGAGACAATTA3980.12385095564393162No Hit
CTCCATTGCTTTGGTGTAGTTCACAAATCGTTTATGGTCCTTTTTCCCAC3880.12073912258755141No Hit
CTATGGTAATCTGGCTAAAATAAAACAAGAGTTTCATATTTCGGGCACAG3860.12011675597627539No Hit
ATCTGGAAGTCACGGTACAGAGAATTACTTAATATGTTAGTAGAGTGGAT3850.11980557267063736No Hit
GAGGGAAACATGGTGGGTGAGAAAAAGATTCGATGAAAATTAGAGGATGT3840.11949438936499934No Hit
GGATATTAGATGGTGGAGACAATTAGTCGACCGCTATGGTAATCTGGCTA3840.11949438936499934No Hit
GTCAAAAGTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGTAT3830.11918320605936134No Hit
ACTCTCAGGTGACTGGTCGGAAATCAGGAGTCCCCCTCAGACAGAGGTGG3790.11793847283680925No Hit
ACCGTACACCATGGCTAGAGAGATGGGCCAGGTCTCGAGGCTTCGCTCAC3780.11762728953117123No Hit
GAAACATGGTGGGTGAGAAAAAGATTCGATGAAAATTAGAGGATGTAAAT3760.1170049229198952No Hit
GATAAAGTTTTCGCGAGAGCGTATGATAAATTCCAGCGAGCAGATCCAGT3750.11669373961425716No Hit
GATGTAAATTGATATTTATCTATGGCTTCATTTTAGTGTCAAAACTCACA3720.11576018969734311No Hit
CATCAGGAGGTTCCATCCAAAGTTCAAGGTTTCTCCTTCCATGAAATGTC3720.11576018969734311No Hit
AGTAAATGGTTTTCTGGGGAGCATAAAGAAGGATATCAAAATAGGGGTGG3720.11576018969734311No Hit
GTCCAAACACTTGTTGGGTGGGTGGTTGGATGGGTGGATGGACGGATGGA3610.11233717333532489No Hit
CAATTAGTCGACCGCTATGGTAATCTGGCTAAAATAAAACAAGAGTTTCA3590.11171480672404886No Hit
TATTTGGGGGGCGGTATGCGGAGATTTATTAAAATTTCAAATTTATCACG3590.11171480672404886No Hit
ATTTAAGACTTACTTAGGAGGTAAGCATGGGCATTAGTAAATGGTTTTCT3570.11109244011277283No Hit
CATTAGTTCAGCTCTATGCTTCTGTTACTTGAGCAAACTTGTTGGGAGAG3460.10766942375075461No Hit
ATCGAAACCTGTGTCCCCGCGGGCATTGTAGCCCGTAAATGGGGGGCTTA3440.10704705713947858No Hit
GTCCTAACCAGGCCATAGGCCAAATGGGGCCTATGAACATATTTGGGGGG3440.10704705713947858No Hit
GTTTAAGGACCTTGGTGCTTCAGAATTACCAAAAGATTTTAGGACAGGAA3380.10517995730565048No Hit
CTCCAGAAACAACGCACTTCTTTCTCAGAGAGGTCTAAAACCTGGTGCTA3380.10517995730565048No Hit
AAGTAGGAGGGCATGGAGTGTGGAGAACACGCAAGCATACTGGGACGATT3370.10486877400001245No Hit
GTTCAGCTCTATGCTTCTGTTACTTGAGCAAACTTGTTGGGAGAGTGTTT3370.10486877400001245No Hit
GTGTATGTCAGTCTCTGCGCTCGCAGTTGTTGAATTTCCTCTCGCAGCTG3270.10175694094363226No Hit
GATGTGAGACGTAACTCTGAATTGTTCTATCTGGAAGTCACGGTACAGAG3240.10082339102671821No Hit
GCTCTATGCTTCTGTTACTTGAGCAAACTTGTTGGGAGAGTGTTTTATGG3230.10051220772108017No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACTA100.0070760003145.086563
GATAAGG100.007089185144.99626145
CTGAACG151.2293608E-4144.99626145
ACCGGTG259.38187E-486.9977659
GGACCAA1800.084.6338356
GACCAAT1850.082.295177
ACCAATC1950.078.0749058
CGAAAGC3100.070.203183
GAAAGCA3300.065.948434
GTTATAT1700.064.008781
TAGGTAT350.00355588362.179964
CGTTGGA350.00355588362.179961
GGCATAG350.00355588362.179961
ATATCCC1750.062.1799554
TATATCC1800.060.452743
GCGAAAG3650.059.6246152
GACAGGC502.0084382E-457.998517
ATCAGGC502.0084382E-457.99851145
GCAGGAC4350.055.0328333
CTTCCCG400.006047780654.373596145
Produced by FastQC (version 0.11.5)