FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_C09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_C09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTCCTGCTCAGCC22.0408163265306123No Hit
GTACTGTTGGTGTACCCATTTTGCAGATGGGGAAACAGAGATAAGACGGT22.0408163265306123No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG11.0204081632653061No Hit
ACCATAAGGTATATAAAACTTATTTCGAAAAGGTAGAAAGGTTGAAACAT11.0204081632653061No Hit
CGTCTCAGTACTACAATGCTGGAACACATCTAATGATCTCACAGATCTCT11.0204081632653061No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCACGCTGTC11.0204081632653061No Hit
GTGTATACTCTGCCTGAACGTAATAATAGCAGATATATTGGTGAACAAGG11.0204081632653061No Hit
CAGGATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTATTCATGATT11.0204081632653061No Hit
CGCTTAGGACTGTGTATTTTTTATTTACACAAGCCTAAAAGCACGGTTGC11.0204081632653061No Hit
AGGTAGTGTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTT11.0204081632653061No Hit
ACACCACGCCCCCTCAGATTGCATGATGGGCCTCTTTGCCATCAGCGGTG11.0204081632653061No Hit
GTTGGATATGATGCCAATGCAACTGTCAAGTGAACCAGTTGAATATCTTA11.0204081632653061No Hit
GGAAAGTGTCGTTGCAGGAAAGAACACAGATCTTGAGGCACTCAGGGAAT11.0204081632653061No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA11.0204081632653061No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA11.0204081632653061No Hit
GGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATCATATAG11.0204081632653061No Hit
ATTACGAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGATGTAA11.0204081632653061No Hit
GAGGGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGATCCAGATTA11.0204081632653061No Hit
ATCTATTAGAAGCGGCCTTATTTTCTCAAATTTCTTCTTTGATGATTCAT11.0204081632653061No Hit
AGCAGGAGGTCTCATCGCAGTATCTGTGTGCCCAAAGATGGGGTTCTTTG11.0204081632653061No Hit
CTTCTAATAGATGGCACAGCATCACTTAGTCCTGGGATGATGATGGGCAT11.0204081632653061No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG11.0204081632653061No Hit
TTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAA11.0204081632653061No Hit
ATTGGGATCAGCTGAAAGTCATCCATGGCTGCACCGGATGCATTGAGCAA11.0204081632653061No Hit
GTGCAATATCCTCAAAGGAAAATTTCAAACAGCTGCCCAGAGGGCAATGA11.0204081632653061No Hit
CACGCAAAGCACAATAGGGAAGAAAAAACAACGACTGAATAAGAGAGGCT11.0204081632653061No Hit
GGCAGAGGAAGAGGGAGAAGGAGAATCCCCAGGAGCAAGGAACCTGATGT11.0204081632653061No Hit
CCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAAT11.0204081632653061No Hit
GGCCCAGGTCACCCAGCTCGGAAGCGCTGGCCGGCCGTGCCGCCGGCCCC11.0204081632653061No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG11.0204081632653061No Hit
CCATTGTTTCAAGGCATGAATTCTCAAATATTCCTGGGTGGGATTCTTCA11.0204081632653061No Hit
TCATAAGAGGAAAGAAAGTGATTCCAAGAGGAAAGCTTTCCACAAGAGGG11.0204081632653061No Hit
CTGTTTCCCCATCTGCAAAATGGGTACACCAACAGTACTTTCCCTCCAGG11.0204081632653061No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG11.0204081632653061No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTAAGT11.0204081632653061No Hit
TCGGAAATCTTCAAAGTAAGAAGCTTTTCAGTCCATAAACCACAATTGAA11.0204081632653061No Hit
GGGCAAGCTTTCCCAAATGTCAAAAGAAGTGAACGCCAAAATTGAACCAT11.0204081632653061No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGGCCCCAA11.0204081632653061No Hit
AGTCAGTGCTAGTCAACACTTATCAATGGATAATAACGACCAGGGAAATT11.0204081632653061No Hit
CCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGC11.0204081632653061No Hit
GAAGTAGACTCCCTACTGAGCAAAGAGCCCGATGTGGGATGCGGGGCTCG11.0204081632653061No Hit
ATATGATGCAATCAAATGCATGAAGAAATTCTTTGGCTGGAAAGAGCCTA11.0204081632653061No Hit
GAAATGAGGCGCTGCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGAT11.0204081632653061No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACAAATTC11.0204081632653061No Hit
CTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGATTATTTGAGAA11.0204081632653061No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCCATGGTGGAACAGATC11.0204081632653061No Hit
ACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAG11.0204081632653061No Hit
TCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGA11.0204081632653061No Hit
TGTTGTGACAGTCTCAGCGCAGGCTCCTCGGCCAGACAGTCTTGGGCCTG11.0204081632653061No Hit
GGCTATTAGTATCAGATGGAGGCCCAAACTTATACAATATACGGAATCTT11.0204081632653061No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACAATGTTAC11.0204081632653061No Hit
ACCCAACAGGATTGGCCCCAAAGATACCACGCCATGGCCTTGCCCCAAAA11.0204081632653061No Hit
GCCCTGAGCCGAAACCAGGAGTCAGAACTGACTGGGCAGCGCCCCCCTGC11.0204081632653061No Hit
TCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAATG11.0204081632653061No Hit
TTTCTTTTCAGCACAAAGGGCTTGAATGAAACCCACCTGCAGGGACAGAG11.0204081632653061No Hit
CCCACTTCTGGAAAAGCACTTTTGCATCATTTTGGAAATGCCTCAAGAGT11.0204081632653061No Hit
GAAAATATAGTAAGTAGATCAGATAAATCTGCGTTATTTATCCGGCAAGA11.0204081632653061No Hit
ACTGGGAGGGAGCCCCTGGAGTATTTCCTCTCAGGGGCTTCTGAGGGAAA11.0204081632653061No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGACCAAATAA11.0204081632653061No Hit
CCAGTATTCAATTACAACAAGGCAACCAAACGACTTACACATCTTGGAAA11.0204081632653061No Hit
TCCTTTATGACTAATGATGTTTCTGTGTACTCTCTCTCTCTCCATCTCTC11.0204081632653061No Hit
GATTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTCTTTACATCAT11.0204081632653061No Hit
GCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAATGAGCAC11.0204081632653061No Hit
AGAGTCTTCAAGATATAATATTACAAAGACAAAGCACCCATAATCCCTTG11.0204081632653061No Hit
TATATGAACTGATAGGGAAAAATTTATCGAATAGATTGCAGCACTTCTGC11.0204081632653061No Hit
TTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCT11.0204081632653061No Hit
TTTCCAAGAGAGATGCTAATGGGTCTGATGACACTGCAGCTCTTCTTACT11.0204081632653061No Hit
ATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCG11.0204081632653061No Hit
GATGAATACAGATTTTGCCAGTAAGGTCCTGCCCACTTTCCCAATAGAGC11.0204081632653061No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11.0204081632653061No Hit
TCATTTTCCTTCCTGTCCTTACGTGGAGGGCATCACTTCTTTCTGTTTGC11.0204081632653061No Hit
GCCCCAAAACTCAGGCAACCTGTCCAACTTGGCCAGGGATGTGGGAGCCT11.0204081632653061No Hit
TTACAGGAACTATGCGCAAGCTTGAAGACCAAAGTCTCCAACAGAACTGC11.0204081632653061No Hit
GTCCAATAGCTGTCACCTAATAACTTCTTCATGTGATGAGGATGTGCATG11.0204081632653061No Hit
GAACTACACCAAAGCAATGGAGTGGGAGGCAGGGAAGAGTAGAGTAGGTG11.0204081632653061No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTGAATTTTCT11.0204081632653061No Hit
TGTTAATATTCACAGCAATGTTATTTATAATAGCCAAAAGTTGGAAACAA11.0204081632653061No Hit
TATTTAATTTCAGTGCTCTTATTAGATAGGCACTCTTATTCAGTCTTTGG11.0204081632653061No Hit
TTCACGGTCCTGTCGCTCTGATAGCCCCTTCGTCCAAGCCAGGAGGGAAG11.0204081632653061No Hit
TTTCTGATGAAATGGAAATCCGAATACATGAAACAGATTCAGCCGAAAAC11.0204081632653061No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATGAACTG11.0204081632653061No Hit
GGCCATTTGACAATTGACAAATAATAAAACGTAGAACATTTGATGCTGAC11.0204081632653061No Hit
CTCCTCTCCACTTAATGAAAAGATGTGAATCTGTGTCTACTCAGATTATA11.0204081632653061No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG11.0204081632653061No Hit
CTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCATACACACAA11.0204081632653061No Hit
TTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCCATCTCC11.0204081632653061No Hit
GGACAACAAAGTGTGCTTGGCCCTTTGGCCAACTCCCCTTCATAGACCCA11.0204081632653061No Hit
AACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGGC11.0204081632653061No Hit
TTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTA11.0204081632653061No Hit
ATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTCT11.0204081632653061No Hit
GTCCCAGGTCACCCAGCTAGGAAGCGCTGTCAGGACTTGACGCAGGCCCA11.0204081632653061No Hit
CACTAAGACCACAGTGGACCATATGGCCATAAACAAAAAGTACACATCAG11.0204081632653061No Hit
GAGCTGTACAGCCGGAGGTTGCCGTTGACTAGTGAGCTGGTACCTGTTAC11.0204081632653061No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATC11.0204081632653061No Hit
CAAGGGGGGTCCAGATTGCTTCAAATGAGAATGTGGAAACCATGGACTCC11.0204081632653061No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAA50.0145.07
TTCTGGA50.0145.06
CCCACTT50.0145.01
ACTTCTG50.0145.04
CCACTTC50.0145.02
CTGGAAA50.0145.08
CACTTCT50.0145.03
TGGACAC50.0145.0145
TGGAAAA50.0145.09
CTTCTGG50.0145.05
Produced by FastQC (version 0.11.5)