FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130855
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3650.067.5571751
GCGAAAG5100.048.349742
CGAAAGC5800.042.514433
CAATTCG3700.041.148069
TACGTCA1204.0566167E-430.210571145
GTATTAC6900.029.4302791
TATCGAC1000.006064721429.0098483
CTAGTAC1804.688278E-628.2040183
GGATAGC2853.2196112E-1027.9919575
TCAATAT7950.027.3580998
GTAGAAA10250.026.1796171
AATATAT8250.024.6144161
GGTCGTT3000.024.166327
TATACTG11100.023.5214985
ATATTCA9100.023.112244
CCTTATA7350.022.6947782
TATATTC9650.022.5465133
TAGAAAC11300.022.4633752
TTATACT9200.022.0727084
CAATATG8700.021.6663559
Produced by FastQC (version 0.11.5)