FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_C06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_C06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163874
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT14450.12415433285733679No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA12720.10929018089587017No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA12650.10868874122112873No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG12130.10422090363733533No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC11900.10224474470604207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2150.074.211831
CGAAAGC3450.050.4523163
CAATTCG3900.046.472549
AGTAGAA8300.043.6898841
GCGAAAG4050.042.9778982
TATATTC8200.039.8004153
ATATTCA8050.038.7401734
GTAGAAA9600.037.7735442
TATACGT1406.5928907E-736.2626044
GACAGTA4200.036.248587
TAGTACC1458.676416E-735.012174
CTACACT3600.034.2480164
TAGAAAC12900.033.170443
AATATAT9200.033.1093331
GTATAAA4150.031.4567171
TCTACAC4650.029.6339533
ATACGTG1753.765108E-629.0100845
CCTTATA4500.029.0100822
TCTATGT4200.027.6286493
GTATTAC9000.027.3984131
Produced by FastQC (version 0.11.5)