FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_C04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_C04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318878
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGACCAATCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACA10080.3161083549194363No Hit
GTACAGAGAATTACTTAATATGTTAGTAGAGTGGATATTAGATGGTGGAG8640.27095001850237393No Hit
TGTTAGTAGAGTGGATATTAGATGGTGGAGACAATTAGTCGACCGCTATG8510.26687322424250026No Hit
CTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAAGCT8440.2646780273333375No Hit
GCTATTAAGGGTAGGCAGGAGAAGATGCATTTAAGACTTACTTAGGAGGT8030.2518204454368128No Hit
ATATTTATCTATGGCTTCATTTTAGTGTCAAAACTCACACACGCGAGGAA7720.24209885912480633No Hit
TCTTTATGCTCCCCAGAAAACCATTTACTAATGCCCATGCTTACCTCCTA7350.23049567546208896No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT7290.22861407811137802No Hit
TCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAGTC7150.2242236842930525No Hit
GCATAAAGAAGGATATCAAAATAGGGGTGGCGAAGGAACTCGTGTTGCAA6840.21450209798104602No Hit
GTACTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAA6730.21105250283807603No Hit
ATCTATGGCTTCATTTTAGTGTCAAAACTCACACACGCGAGGAAGCGTGT6460.20258531475987682No Hit
CCCCAACCCCACGCACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGA6450.202271715201425No Hit
AAATTAGAGGATGTAAATTGATATTTATCTATGGCTTCATTTTAGTGTCA6440.2019581156429732No Hit
GAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAGTCATA6280.196940522707744No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTG6230.1953725249154849No Hit
CCCTGAAAGTGTGCCTTCTCCTCTGGGCCTCATCACAGTTCACGGTGCCA6000.18815973507109301No Hit
AATCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAG5960.18690533683728572No Hit
CCTCTGGGCCTCATCACAGTTCACGGTGCCAGTACTCTCAGGTGACTGGT5620.1762429518499238No Hit
GCTTTGGTTCCCGCCCACAGTGTCAAAAGTCATAACTCGAGCGAGCAGAT5240.16432616862875457No Hit
GTGCCAGTACTCTCAGGTGACTGGTCGGAAATCAGGAGTCCCCCTCAGAC5160.16181737216114No Hit
CTATGAGCCCAGGCGGTGAGTACTATTATTGTTCCCATTTTACAGTTGAG5110.1602493743688809No Hit
ACTCTACTCTTCCCTGCCTCCCACTCCATTGCTTTGGTGTAGTTCACAAA4970.1558589805505554No Hit
CTCCAGAGGCGCCCTACTCACTCGCTTTCAGGTTTGTCCAGCTAGCCAAA4910.15397738319984444No Hit
ATATTAGATGGTGGAGACAATTAGTCGACCGCTATGGTAATCTGGCTAAA4790.15021418849842258No Hit
CACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGAAAGTGTGCCTTCT4760.14927338982306712No Hit
GTTATATCCCTGCATCACGGCCATTCAATAGGCCCTGCTCAGGTCATTCT4630.14519659556319345No Hit
GTCTAAAACCTGGTGCTAGGTCACAGAGCTTGTCACAAGAGAGGCAGGAT4530.14206059997867523No Hit
GTGTCAAAAGTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGT4460.13986540306951248No Hit
GGTTAGGACATCTGGTCTGTGGGTCTGGACTCCGAGTGCCAGGGTCTGAG4460.13986540306951248No Hit
GTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGTATGATAAAT4420.13861100483570518No Hit
CCGTAGAAGCTCCTGAAGAATGTCTTTCAGTCTCTGTCTTACTGGACCAC4380.1373566066018979No Hit
GGCATGGAGTGTGGAGAACACGCAAGCATACTGGGACGATTGAGTGTTGA4350.13641580792654243No Hit
GACATAATGTGAGTAAAGCCCTCATCACCTGCATGGCTGCTATGATAAGC4320.13547500925118697No Hit
GTCCAGGAGGTCTGTGCTCCAGGAGGTCTGTGCTCCATCTTCACGGAGGA4310.13516140969273516No Hit
CTACTAACATATTAAGTAATTCTCTGTACCGTGACTTCCAGATAGAACAA4300.13484781013428335No Hit
CCCTCAGACAGAGGTGGAGGTGGCAAGCACGCGGCACCTACTCTACTCTT4270.1339070114589279No Hit
CTTAATAGCTGTGCCCGAAATATGAAACTCTTGTTTTATTTTAGCCAGAT4240.1329662127835724No Hit
ATGGTAATCTGGCTAAAATAAAACAAGAGTTTCATATTTCGGGCACAGCT4170.13077101587440965No Hit
CCCCTATTTTGATATCCTTCTTTATGCTCCCCAGAAAACCATTTACTAAT4070.12763502028989143No Hit
GCTTTGGTGTAGTTCACAAATCGTTTATGGTCCTTTTTCCCACTGAACTC3990.12512622382227684No Hit
GAATTACTTAATATGTTAGTAGAGTGGATATTAGATGGTGGAGACAATTA3970.12449902470537322No Hit
ATTGAGTGTTGACCGCGAGACAGAATGTCTGCAGAAGGTGGGAAGATTGC3960.12418542514692138No Hit
CTCCATTGCTTTGGTGTAGTTCACAAATCGTTTATGGTCCTTTTTCCCAC3860.12104942956240317No Hit
ATCTGGAAGTCACGGTACAGAGAATTACTTAATATGTTAGTAGAGTGGAT3830.12010863088704771No Hit
CTATGGTAATCTGGCTAAAATAAAACAAGAGTTTCATATTTCGGGCACAG3830.12010863088704771No Hit
GAGGGAAACATGGTGGGTGAGAAAAAGATTCGATGAAAATTAGAGGATGT3810.11948143177014407No Hit
GGATATTAGATGGTGGAGACAATTAGTCGACCGCTATGGTAATCTGGCTA3810.11948143177014407No Hit
GTCAAAAGTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGTAT3770.11822703353633678No Hit
ACTCTCAGGTGACTGGTCGGAAATCAGGAGTCCCCCTCAGACAGAGGTGG3730.1169726353025295No Hit
GAAACATGGTGGGTGAGAAAAAGATTCGATGAAAATTAGAGGATGTAAAT3730.1169726353025295No Hit
GATAAAGTTTTCGCGAGAGCGTATGATAAATTCCAGCGAGCAGATCCAGT3730.1169726353025295No Hit
ACCGTACACCATGGCTAGAGAGATGGGCCAGGTCTCGAGGCTTCGCTCAC3730.1169726353025295No Hit
GATGTAAATTGATATTTATCTATGGCTTCATTTTAGTGTCAAAACTCACA3710.11634543618562584No Hit
AGTAAATGGTTTTCTGGGGAGCATAAAGAAGGATATCAAAATAGGGGTGG3710.11634543618562584No Hit
CATCAGGAGGTTCCATCCAAAGTTCAAGGTTTCTCCTTCCATGAAATGTC3680.11540463751027039No Hit
GTCCAAACACTTGTTGGGTGGGTGGTTGGATGGGTGGATGGACGGATGGA3610.11320944060110763No Hit
CAATTAGTCGACCGCTATGGTAATCTGGCTAAAATAAAACAAGAGTTTCA3580.11226864192575216No Hit
TATTTGGGGGGCGGTATGCGGAGATTTATTAAAATTTCAAATTTATCACG3580.11226864192575216No Hit
ATTTAAGACTTACTTAGGAGGTAAGCATGGGCATTAGTAAATGGTTTTCT3550.1113278432503967No Hit
CATTAGTTCAGCTCTATGCTTCTGTTACTTGAGCAAACTTGTTGGGAGAG3430.10756464854897484No Hit
GTCCTAACCAGGCCATAGGCCAAATGGGGCCTATGAACATATTTGGGGGG3400.10662384987361938No Hit
ATCGAAACCTGTGTCCCCGCGGGCATTGTAGCCCGTAAATGGGGGGCTTA3390.10631025031516755No Hit
AAGTAGGAGGGCATGGAGTGTGGAGAACACGCAAGCATACTGGGACGATT3380.10599665075671574No Hit
CTCCAGAAACAACGCACTTCTTTCTCAGAGAGGTCTAAAACCTGGTGCTA3360.10536945163981208No Hit
GTTTAAGGACCTTGGTGCTTCAGAATTACCAAAAGATTTTAGGACAGGAA3340.10474225252290845No Hit
GTTCAGCTCTATGCTTCTGTTACTTGAGCAAACTTGTTGGGAGAGTGTTT3320.1041150534060048No Hit
GTGTATGTCAGTCTCTGCGCTCGCAGTTGTTGAATTTCCTCTCGCAGCTG3240.10160625693839023No Hit
GATGTGAGACGTAACTCTGAATTGTTCTATCTGGAAGTCACGGTACAGAG3230.10129265737993841No Hit
GCTCTATGCTTCTGTTACTTGAGCAAACTTGTTGGGAGAGTGTTTTATGG3220.1009790578214866No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTAC100.007085885145.018681
ACTAGCC100.0070892083144.995938
TGTATAG259.3760464E-487.011192
TATTGTA259.3760464E-487.011192
GACCAAT2100.086.30717
TAGATAC353.431487E-582.867815
GGACCAA2250.080.565926
ACCGTAC851.8189894E-1276.774591
GTATGTA406.651988E-572.509341
GAGCGAA406.651988E-572.509341
TAGGTAT300.001933541572.509336
CGAAAGC3350.071.42713
ACCAATC2600.069.709588
AGCGTCC754.836693E-967.675385
GCGTCCT754.836693E-967.675386
GGACGAA1009.094947E-1265.25841
ATGTAGT451.1918567E-464.4527363
GTAATAA451.1927822E-464.442638
CAGCGTC808.054485E-963.445674
ATAGGTA350.00356245962.1508565
Produced by FastQC (version 0.11.5)