FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATTTTGGACAGTATGGATAGCAAATATTAGCATTGAAACAACTAC12.127659574468085No Hit
ATGCTATAAATTACAACCTGTCTCCAATAAGAGATCCTAGGCAAAAAATT12.127659574468085No Hit
AGGTACTTTTTTGGACAGTTTGGATAGCAAATAGTAGCAATGCCACAAGT12.127659574468085No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTGT12.127659574468085No Hit
GCCCTACGTCGCCTTGCCCACTTAGCCCAGTAGCCTTCGCATCTCTTGTC12.127659574468085No Hit
CAATATGGAGAGAATAATAAAAATTATTAATATAATTTAGAAGACCCCCA12.127659574468085No Hit
CGCCAAAGCAGGTACTGATCCAAATTCGAAGACATTGAGCGACAATGCTT12.127659574468085No Hit
GTAGAAACAAGGTCGTTATTAAACAATTCGACACTAATTGATGGCCATCC12.127659574468085No Hit
AAGCAGACTTCAGGAATGTGAAGATTCCGTATATTCTATAAGTATGATAC12.127659574468085No Hit
CCATCCAAATTTGCACGGACATCAAACTCATTTATTATGATGGCCTAAGA12.127659574468085No Hit
GCATTTTTTCATGAAGTACAAGTTAAATTAATTATTGTTGCCGTCTGAGG12.127659574468085No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA12.127659574468085No Hit
TTTGGAAGTTTGTTTCATGTATTCGGTTTTCCATTACATCGACGAACGTA12.127659574468085No Hit
GCAGTTCAAAGATATTCAATATTGAGAGAATAAAAGAACTGAGAGAGCTA12.127659574468085No Hit
GATAGTCACGCAAGGGGGGAGGGCGAAGCCGTCGGGGGTCTAGGCGCGAT12.127659574468085No Hit
TCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGA12.127659574468085No Hit
ATCCACCTACTCCTGCTGACAAACAAAGTAACAATGAGAAAGCACAAGCA12.127659574468085No Hit
AGATGGCTCCGAAGTCAAAAAAGAAGGGGCACCCCGGGAGGGAGCAGAAG12.127659574468085No Hit
GCATAAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC12.127659574468085No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGCTTAGCTCATCTTT12.127659574468085No Hit
GAATAAAAGAACTGAGAGATCTAATGTAGCAGTACCTCACTCTCGATATA12.127659574468085No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA12.127659574468085No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG12.127659574468085No Hit
TGTTAGTAGAGTGGATATTAGATGGTGGAGCCAATTAGTCGACCGATATG12.127659574468085No Hit
AGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATAGAACAA12.127659574468085No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC12.127659574468085No Hit
ATGAAGGACAAGTTAAATTCATTATATTTGCGGTCTGAGTTCTGCAATGG12.127659574468085No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC12.127659574468085No Hit
CCTTTGTGCGACAATTCTTCAATCCAATTCTGGTAGAGATTCAGAAAATG12.127659574468085No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC12.127659574468085No Hit
TCTCACAGGAGGATTCCATGACCAAGGCAGATAGTGGCGATCTGAACTTT12.127659574468085No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA12.127659574468085No Hit
GGAGCAAGAGAGAGAGGGAGAGAGAGAGAGGGAGAGAGAGGGAGAAAGAG12.127659574468085No Hit
ACCCTAGTCTTGCCATTTTGTTTGAGAACATTATAGGTCCCATGCTCAGG12.127659574468085No Hit
GTTTTGGGTACTCTGAGTATTTAGGTGTGTCATAAGTCCCATTATTGAAT12.127659574468085No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGAAACACAAATTCAGACGA12.127659574468085No Hit
GGGCGGCGCTCCGCGCCCGCGGCGGTCGTGATCCGGCCAGACGCGCAGGC12.127659574468085No Hit
ACTTTGTGCGACAATGCTGCAATCCAATGATCGTCGAGCTTGCGGAAGAG12.127659574468085No Hit
GAAAGAATATGGGGAAGATCCCAAAATCGAAACTAACAACTTTGCTGCAA12.127659574468085No Hit
CGCAACAGAAGCACCAAGCACTCAGAATGAAGTGGAAGATGGCAATGACA12.127659574468085No Hit
GCATTAATCATCAAAGAACTGAGGATTATTGAAAAACGAAGGATTCTACA12.127659574468085No Hit
GAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGA12.127659574468085No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTATGCTGCAATATGCA12.127659574468085No Hit
CCCTAGCCCACATATGCTTCCAGCTTCCAAACCACTCAGACACGACAACT12.127659574468085No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTTGTAATGCCAGCCCAT12.127659574468085No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCG12.127659574468085No Hit
GGAAAGTGTGCAGGACCTTCCTGGCAAAATCTGTATTCAACAGTCTATAT12.127659574468085No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)