FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_C01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_C01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076506
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4200.055.262451
CAGCTCG3900.050.191129
GCGAAAG5400.042.9759182
ATCTATG1601.0077201E-940.7935452
CTATACC2250.038.678324
TCTATAC2600.036.2609333
TATATAC9300.035.0912253
TAGCCGG1301.6514869E-533.471636
TACACCG1301.6551665E-533.4607477
TATACTG7950.032.8400885
GTTATAC4850.031.4012183
GTAGCCG1453.483463E-530.0090485
ACCGTAC1255.1548134E-428.9993138
GTATATA8200.028.3051591
GTATTAC2603.7034624E-927.8969141
ACCATGT5200.027.8839578
TTATACT10800.026.859954
TACTCCG1358.0688915E-426.859955
GGTGTAC2507.798553E-826.111511
GTACGCT1450.001226673524.9994097
Produced by FastQC (version 0.11.5)