FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_B12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_B12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247623
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG20120.16126666468957368No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT16730.13409499504257297No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT15900.12744234436203886No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15050.12062938884582924No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT14580.11686222520745448No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT14000.1122133849728644No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC13850.111011098705298No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG13730.11004926969124487No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA13070.10475921011395269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1850.086.2508541
CCTTATA4750.044.2720682
CGAAAGC3850.041.4369133
TCACGCG700.001056112541.43359145
TTATACT6150.036.552074
GGTCGTT3650.035.752027
GTAGAAA14300.035.5037731
GCGAAAG4700.035.4858672
AGTAGAA9450.033.0026741
AGGTCGT4200.031.0776826
CAGCTCG4600.029.9444779
CAATTCG5450.027.9346859
GTCTAAG7600.027.6755871
ATATTCA7400.027.4379524
GCAGGTA4650.026.5107122
CCCTTAT6850.026.4704861
CAGGTAC4950.026.3689423
GGTAGTT3751.0913936E-1125.1323477
CAAGGTA4051.8189894E-1225.0667724
TAGAAAC19850.024.841272
Produced by FastQC (version 0.11.5)