FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_B05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_B05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333595
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGAGCGGTGGCCACTTCCCTCAGAAACTGTCGATGCCTCTCAGAA19030.14269699571459102No Hit
ATATATATCTTTAGAGTCAAGAAAAGGCATCATTGCATTAGCAAAAAGGG19030.14269699571459102No Hit
GTATAAAGTCCCGCCCAAAACTGTACCACAGAAAGAAAGCTGGGTAACTT17660.13242401178768667No Hit
GTCCTGGATGACCTCTTGGGAAGTTACCCAGCTTTCTTTCTGTGGTACAG17520.13137421780975483No Hit
GGTTAAGAGAGTGGCCTCTTACTGTTTGCTTTTTGAGTCCTGCACATGTA15980.1198264840525047No Hit
GTGCAGGGACAGTCCGAGGTGCTTCCTGGGGAAAGAGAAAGGTGAACAAA14250.10685402989663279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA851.8189894E-1276.777893
TTGCGCC1150.063.04721145
GTCACGG604.9414934E-448.3416334
CAATTCG1650.048.3325739
TCAGCGC1307.350536E-944.618027145
TACTAGA1351.0248186E-842.970342
GACATCG1201.9743493E-742.2909977
AGCGAAA7650.041.7080731
TATTAGG2300.040.98532
GACGTAT1451.927765E-840.00237145
GTATGTA2252.0008883E-1135.4518621
CGAAAGC9150.034.8693733
CCTTACC3750.034.8059732
CCCTTAC4650.034.3082541
TATAACC2551.8189894E-1234.1235054
CTATCCG2353.45608E-1133.9419984
AATCTCG1501.1327465E-633.8391465
GGGCGCT2154.8567017E-1033.7267236
TAATACT3150.032.2277574
ATCCGGA2507.094059E-1131.905486
Produced by FastQC (version 0.11.5)