FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003832
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA27760.2765402975796747No Hit
GTATTTATGCTGTAAGATGTCTGGACTGGAAAGTACTAATCGGAGGGAAC16140.1607838761864535No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA13870.13817053052702044No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG13320.13269152607209175No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT11610.11565680313040429No Hit
CTTAAGGACCATCTGAATGTGAAGACTCGAGAATTATTTGCCGATGGATT11380.11336558308561592No Hit
GTACTAGTCCCTGCTGCGTCAGCCTACCCTACTGCCAGAGTAGTCTCTTC11110.11067588998956002No Hit
ATTTAAGGTCAAAGGGGCAACTCAAGGTCAAGTATCTGAATAACTAGCAG11050.11007818041265868No Hit
CCTAAGGAAGTGAGGGGGAAAGTTACGATAGGAAAAGAAGAGTGGCTATT10950.10908199778448983No Hit
GAAAAGAAGAGTGGCTATTGCACGGACATGCTTGGGCAAGACCGGAGGAA10840.1079861968935041No Hit
ACGTTATTGTATGTCTGTGGGTGTAAGCAGCTGTGCCAATCCTTCTGGAA10450.10410108464364554No Hit
ATGTATTGCCTGCTCATTGCATTTATGTAGCACCTAAGGAAGTGAGGGGG10260.10220833765012473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4450.066.829231
TATAACG350.00355985762.1721424
GCGAAAG6100.049.941552
TATACGG450.00962122448.3561064
ATTCGCC1602.0008883E-1145.333852
TACCGTA803.7352627E-545.311277
GGTCTAC1505.347829E-1043.5204961
CAGCTCG6050.043.1393289
TATACCG855.326682E-542.6671565
GGAACGC907.481463E-540.27869145
TATACAG7200.039.2893375
CATACAT5000.039.1489378
GATTCGC1951.6916601E-1037.197011
TCGAATA800.00203639236.250824145
TAATCTA5000.034.8163955
ATATACC1051.848596E-434.5400774
GGATATA850.002735664434.1337241
GTATAAT3250.033.4773061
CTATGCG1301.6494609E-533.4773034
CGGGTGT1102.4365443E-432.953658
Produced by FastQC (version 0.11.5)