FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_A03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_A03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121128
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2550.051.189221
CAATTCG3550.049.0109949
TAATACG750.001481972338.674584
TAGGCCG750.001482622238.671127145
CAGCTCG5550.035.2680479
CGAAAGC4200.034.5308723
TTAGACG1102.4334124E-432.961294
TTATACC3700.031.3577654
GCGAAAG4500.030.6173732
TACCTAT5000.030.4480827
ACCTATC5100.029.8510598
CCTCGCG1753.7717637E-629.003345145
GTAGAAA10150.028.5785521
GTAATAC6100.028.5304283
TAGACGA1050.0077011527.6246975
TATATTC6900.027.324433
TAGACCT4150.026.2101784
TATATAC16650.025.2604223
CTGATCG1450.001225711225.002886145
AATATAT7700.024.4866181
Produced by FastQC (version 0.11.5)