FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_A02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_A02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996238
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2000.0101.521091
ACCGTAT350.00356711162.1401678
GCGAAAG3600.056.4006041
TCTAGCG1600.049.8516042
GTAGGTA2900.042.498168
CTAGCGT1859.640644E-1139.1953623
CGCCGCG951.02763E-438.16582145
TAAAGTA3400.036.2557144
ACGGCCC1406.60144E-736.255714
CAACTCG850.002739188534.124737145
CGAAAGC5950.034.123022
TAATACC4500.033.8386654
ATACCTA4400.031.3133226
GTCTTAA3500.031.0778831
GTGTAGG2701.70985E-1029.5431731
GTTAGCC1000.00606977329.0045683
TAACACA4100.028.2971384
ACGAAAC1306.4845703E-427.883418
CCGGACT3453.6379788E-1227.324516145
GTAGGGC3555.456968E-1226.5534783
Produced by FastQC (version 0.11.5)