FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R1_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R1_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGATAAGGCATTGAAAGTATATCTGCATGGTCGGAAAATCGGAACTA12.7777777777777777No Hit
CAATGAGACATATGTCATTTGTAGTAGACGAAAAGGGTGAAACAACAACA12.7777777777777777No Hit
AAAATAAGGCCTCTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGAT12.7777777777777777No Hit
CATATAGACTGTTGAATACAATTATTGCTTCACCCCGTTCGTCGATGAAA12.7777777777777777No Hit
GTGTGCCACTTGTGAACAGATTGCTGATTCACCGCATCGGTCGCACAGAC12.7777777777777777No Hit
GGTTCCCCACCGAACAAAAGCCGATGAAGGGACTTGATCCCAGGACCTGA12.7777777777777777No Hit
CCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCAC12.7777777777777777No Hit
ATTAATGGAGTAACAGTGATAAAGAACAACATGATAAAAAATGACATTGG12.7777777777777777No Hit
GCAGTGACATTAAATACGATTCCCAAAGATGCAGAGAGAGGCAAGTTAAA12.7777777777777777No Hit
GATGCTTCAATAGTGCATTCGGGTCACCAGATTCTACAATTATTGATTCA12.7777777777777777No Hit
GAGATACCAAAATACAGCAGAAAAGAGAATAATGGACAACGCCCCAGACT12.7777777777777777No Hit
ATTGAATCCATCGACAATGTGATGGGAATGATCGGAATACTGCCCGACAT12.7777777777777777No Hit
CAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGG12.7777777777777777No Hit
AGTAGAAACAAGGTACTTTTTTGTAAAGTATGGATATCAAATAGTAGCAT12.7777777777777777No Hit
ATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTT12.7777777777777777No Hit
GGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTACTGGCGA12.7777777777777777No Hit
CACTAACACCACTCTGCACCAAAACGCAATAATCAAAAAGAACCCATAAG12.7777777777777777No Hit
GCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGAGAACCAATGG12.7777777777777777No Hit
CATAAAACACAAAAAACCAATACTAAGCAAAGGGAAACTGTAATTTTTAA12.7777777777777777No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCAGCACT12.7777777777777777No Hit
GGCCTGTATTAGCTCTCGCCACCTGCTTGAAAACCCTCATTGGTTCTTGC12.7777777777777777No Hit
GACAATGGATTTTTTTATTCACCATTCATTGATAGTCTACTCCGATATAA12.7777777777777777No Hit
AAACCCAGGGATCATTAATCAGGCACTCCTCGAATGCTGCATATAGCCCC12.7777777777777777No Hit
AATTTACATGACCCATGTCATATTGATATTGGAGTGTCCATTGGTTAGAA12.7777777777777777No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC12.7777777777777777No Hit
CCTATATCAACACCTAGGGAAAAAATTCTCGAAAACAGTGCAGCCCATCT12.7777777777777777No Hit
GAGTGTGTCTGTGTTCAAGGAAGAATGAGGAGGCCACTGTGGCTGGAACA12.7777777777777777No Hit
ATCACATCCTGTGCCTCCTTGGCTCTGAGATCTGCATGGGCAGGGTTTGT12.7777777777777777No Hit
TATGGAGAGAATAAAAGAACGGAGAGATCTAATGTCGAAGTCCCGCACTC12.7777777777777777No Hit
CACAAGTAGATGGAGAGGCAGGCAGAGAGAGAGAGAGGGGGAAGCAGGCT12.7777777777777777No Hit
GAATGTCTTCATGCATTTGATTGCATCATATTGTGGTATTCCCTCCCCCT12.7777777777777777No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACA12.7777777777777777No Hit
GTATGGATAGCTAATATAAGCATATCCACAACTACTTCAGTGAATCTGTG12.7777777777777777No Hit
GATCGGAATAATGCACGCCATGACCAAAATCCCGGAGATGGCTCTGAGAT12.7777777777777777No Hit
TACTTTTTGATTATGGCCATATGGTCCACACTGGTCAAATTTCTATTATA12.7777777777777777No Hit
GTAGGAGTCTATGGGATTCCTTTCGTCTGTCCGAAAGAGGCGAAGAGACA12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)