FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCGATTGTCTCCCGCAGAAGTAAGTGAAACGCAAGGACCTGCGACGTT15.88235294117647No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC15.88235294117647No Hit
TTTCTGAGTATTGGTGTGTTCTGTTTACTGTTTCCATGGTTTATCCTGTG15.88235294117647No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC15.88235294117647No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA15.88235294117647No Hit
GCATTAAGCAAAGCAGTATTAATGTATACCCCCTTCATTATATATTCTGT15.88235294117647No Hit
GACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCGG15.88235294117647No Hit
ATTTGAATGGATGTCAATCCGCCTCTACTTTTCCTAGCGCAAAATGCCAT15.88235294117647No Hit
TGCGGGAAAGGCAATGAAAGCATATGGGGAAGGTCCGAAAATCGAAACTA15.88235294117647No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT15.88235294117647No Hit
GATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACA15.88235294117647No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA15.88235294117647No Hit
GCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAA15.88235294117647No Hit
GATAAAGAACAACATGATAAACAATGAACTTGGACCTGCAACGGACCAGA15.88235294117647No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT15.88235294117647No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG15.88235294117647No Hit
ACGTTGTGCGACAATGCTTCTCTCCAATGAGCGTCGAGCTTGCGGGAAAG15.88235294117647No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)