FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901574
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG90701.0060183634399396No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA79000.8762453220700686No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA70270.7794146681248573No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT67450.748136037640837No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG53380.5920756366088641No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG53170.589746376891969No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC52660.5840896032937951No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA51450.5706686306393042No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG42420.47051046281281406No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC42290.46906854013092664No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40390.44799428554949455No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT38260.42436893699241546No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT38010.42159600875801656No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC35440.3930903065083953No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC34280.38022391950078416No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC31640.35094179734553127No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG30690.34040467005481523No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA29860.3311985483166107No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT29630.3286474543409637No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG29610.32842562008221177No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT27560.3056876085601404No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG27360.30346926597262125No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC27330.30313651458449337No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA26040.2888282048949948No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT25790.2860552766605958No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG25540.2832823484261969No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA25050.2778474090867749No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC24710.27407622668799236No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG24580.2726343040061049No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATATT23810.2640936850441561No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG22900.25400022627094393No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG22020.24423951888585962No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT21860.2424648448158443No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC21550.23902641380518957No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA21470.23813907677018192No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG21330.2365862369589185No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA21090.2339242258538955No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20460.22693644670321017No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA20080.22272159578692377No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC20020.222056093010668No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC19960.22139059023441227No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG18710.20752594906241748No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA18220.20209100972299557No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA17750.19687790464232555No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC17480.19388314214917468No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA17110.18977920836226422No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT17000.18855911993912866No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA16830.1866735287397374No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT16570.1837896833759625No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC16200.17968574958905204No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA16160.1792420810715482No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT15360.17036871072147156No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG15070.16715211396956878No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC14780.163935517217666No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA14140.15683682093760468No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG14140.15683682093760468No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA13800.1530656385388221No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT13710.15206738437443848No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA13680.15173463298631062No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC13470.1494053732694155No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA13390.14851803623440782No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC13350.148074367716904No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG13160.1459669422587608No Hit
GGATATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGTTGGA12930.14341584828311377No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT12600.13975558301370714No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC12410.13764815755556392No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG12290.13631715200305244No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA12100.13420972654490923No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA11890.1318804668280141No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT11670.12944028998174303No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA11600.12866387007611133No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT11350.12589094184171237No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA11350.12589094184171237No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA10820.1200123339847866No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10790.11967958259665873No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC10690.11857041130289914No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA10530.11679573723288382No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG10430.11568656593912423No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA10420.11557564880974827No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA10070.11169354928158975No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA10030.11124988076408593No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA9740.10803328401218312No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC9700.1075896154946793No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG9630.10681319558904762No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG9330.10348568170776885No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT9230.10237651041400929No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC9220.1022655932846333No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA9200.10204375902588139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA13200.0113.69711
GCAGGTA4750.0109.898532
AGCGAAA2950.0105.681611
CAGGTAC5100.0103.7780763
CCGCACG650.0100.38808145
GGTACTG5450.097.1134345
CGAAAGC3450.090.365433
GCGAAAG3600.086.6002042
ACTGATC6500.080.306018
GAAAGCA4050.076.977964
AGCAGGC3300.076.896583
AAATATA12250.073.982145
GTACTGA7300.073.495686
AGAGTTA300.001935416372.502495145
CTTACAC406.661696E-572.5024953
TCAAACG300.001935416372.5024953
GTAGAAA21450.070.981472
AGGTACT7850.070.19354
GGTCAAA12950.068.303511
TATATTC21250.065.849323
Produced by FastQC (version 0.11.5)