FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTTATAGTGAATGAACTTTTAAAACATAACGTGTTGGCAGAAAACAA50.16728002676480427No Hit
TTACTGCAAAGCCCGAGAGTTGGGCTTAAATTTGAATGAGGTTGTACTGA30.10036801605888257No Hit
GGATTAAAGTTACTAACAAATAAGAAGAACAAGGAAAATAACTCCTAGAT30.10036801605888257No Hit
GACATGAACATGACAAACCTGAGATGTTGGAAGAAGAAACTCTAGATAAA30.10036801605888257No Hit
ACCCAGGGAGGTCAATAAAGTTTGAAGATTCTTAATTGACTTGATAAGGT30.10036801605888257No Hit
GAAGAGAGGAAGGAATGTGCAAGAGAAGGGAAGGCTTTGAAAGGATGAAG30.10036801605888257No Hit
GCTATGAAGTGTATACCTACTCACTGGCACAGACCTGTCACTCATCAACC30.10036801605888257No Hit
CCACTAAGAGTATCCCCAAGTCGCACTGAAAGTTTCTTACCAAAAGCTTT30.10036801605888257No Hit
ATAATTACTGTGTAGCCTGTTACATATTTACTGATTTATTAATCTTTCTT30.10036801605888257No Hit
AAGTAGTGTAGAGGATGGCTTGGAAAGGGAGGGACTGGCCGTACAAAAAG30.10036801605888257No Hit
GACAAATACTGTCCTCATGCTGATATGACTTCATAGTCAAATTTATAGAG30.10036801605888257No Hit
TCTGAGATGTGTCTGAAGAAGCTATCAGGTTCTTTTAAACAAAAGGAAAC30.10036801605888257No Hit
CTACAGAGCAGGGAGCCAGATGTGGGGCTCAATCCCAGGACTCTGGGATC30.10036801605888257No Hit
ATATTAATAAGTGTGGAGACGGGGTGAACGGGGAAGCACGTACGTAGGAA30.10036801605888257No Hit
GTGTAGAGGATGGCTTGGAAAGGGAGGGACTGGCCGTACAAAAAGTTCAC30.10036801605888257No Hit
CTCAATGTTTATAGTGAATGAACTTTTAAAACATAACGTGTTGGCAGAAA30.10036801605888257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGGC100.0067389184145.03
Produced by FastQC (version 0.11.5)