FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063427
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTAAGAGTATCCCCAAGTCGCACTGAAAGTTTCTTACCAAAAGCTTT11120.10456759138144885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGC755.638867E-1177.332739
TATGCGT400.00604330154.3924832
AGCGAAA7400.048.022191
CGAAAGC8150.045.3826833
TAGCGCG750.00148245738.67182145
ACGCACG800.002035340836.254833145
TCGCGTT1001.3904177E-436.254833145
GCGAAAG10150.035.0112532
GACCGTT2351.2623786E-930.8508228
TATAGAC3550.030.643652
TATTACG950.004720001530.536132
TACGCAG5350.029.8226685
GTTACGC5500.029.0093233
TTACGCA5600.028.49134
TATACAG5700.027.9914515
CAATCGG2405.2654286E-827.191126145
TAGGCTT4300.026.9854165
TATAGTA1658.400675E-526.3721125
ACCATCG2207.487233E-726.363438
CGTGCAA1100.00967222226.363439
Produced by FastQC (version 0.11.5)