FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGCTTGGGACATTTGACACTGTCCAAATAATAAGACTTCTCCCCG13.571428571428571No Hit
CATCCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCA13.571428571428571No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA13.571428571428571No Hit
TCTGAGCCCACTGAATTTTAACAGTCTCCCAGTTGCTGATGCTCGACTGA13.571428571428571No Hit
ATCTTTACAGTCGTCAAAGTCGACCGTATCTTGTGCCAAGTTCTGACCAA13.571428571428571No Hit
CTGCTGGAACCCGGGGACAAAATAATATTTGAGCCAAATGGAAATCTAAT13.571428571428571No Hit
GTCAGCATCCACAGCATTCTGCTGTTCCTCTCGATATTCTTCCCTCATAG13.571428571428571No Hit
GTGCTTGAGGCGATGTCATTCGATTTGTCAGACCCTTATAGCAATTATTT13.571428571428571No Hit
CATCTGGGTCTTCAGTTAAAGTGCCAGCATCCTTTCCGAGAACTGTGAGT13.571428571428571No Hit
GGCATGGACACTTGTAAACAGTATTTGCAACACCACAGGAGCTGAGAAAC13.571428571428571No Hit
AACCGAGGTCGAAACGTACGTTCTCTCTATCGTCCCGTCAGGCCCCCTCA13.571428571428571No Hit
TAGTATTGGTAATGAAACGAAAACGGGACTCTATCATACTTACTGACAGC13.571428571428571No Hit
GCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACGGAAGC13.571428571428571No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGAGGATTTCC13.571428571428571No Hit
GATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG13.571428571428571No Hit
CCTAAGTTAGACGGGTGATGAACACCCCATAGTACAAGGACTTCTTTCTC13.571428571428571No Hit
AGGAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT13.571428571428571No Hit
CTCACTAAGACCACTGTTGACCATATTGCAATAATAAAAAAGTACACATC13.571428571428571No Hit
GCATGTGTGAGGAAGGACTTGAACCAACAAGCATTAAGCAAAACCCATGG13.571428571428571No Hit
TAGCTAGGTGATGCACCATTCATTGATCGTCTTAGACGATATCATAATTC13.571428571428571No Hit
GACTTCTTCAATCGTGGCACAGAACATCATGATCTCAGAGCACTCATAGT13.571428571428571No Hit
CAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAG13.571428571428571No Hit
ACTTATGATTGGACATTAAACGGAAATCAACCGGCAGCAACTGCATTGGC13.571428571428571No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTATTCTTTCTTG13.571428571428571No Hit
TGCCTGGCCTGGCTAGCACCCTCCATGGCCTCTGCTGCCTGCGCACTCGA13.571428571428571No Hit
GATGCATATTATCAACTGCAAAGACACATGAGACCCCATAAAGAAACTGA13.571428571428571No Hit
GTCTAATATTGCAAATAGGAAATATTGTTTCAATATGGCTCGGCCACTCG13.571428571428571No Hit
ACTCAGCTTCAATCATACTCTCTATTTGTTGAAGTGATTGGAGGAGGCAA13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)