FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAAATGTCCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTG11.282051282051282No Hit
TGTAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA11.282051282051282No Hit
AGGCAGATGGTACATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAG11.282051282051282No Hit
GTCATGGCGTCCCAAGGCACCAAACGGTCTTACGAACAGATGGAGACTGA11.282051282051282No Hit
TCTTTCAGTTTTGGTGGGTTCACTTTCAAAAGGACAAGCGGATCATCAGT11.282051282051282No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCC11.282051282051282No Hit
GGAATGTTTGTCATTTAGCAAGGCCCCTTGAGTCAAGAAGAAGGTTCTGC11.282051282051282No Hit
GAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA11.282051282051282No Hit
ACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCAA11.282051282051282No Hit
GTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTC11.282051282051282No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT11.282051282051282No Hit
GGAAAGAACACAGATCTTGAGGCTCTCATGGAATGCCTAAAGACAAGACC11.282051282051282No Hit
GGTCTAGACCGTTTCCCTGATCTTGCCATATTATGTTTATCAACATGAAC11.282051282051282No Hit
TATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGA11.282051282051282No Hit
CATATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAAC11.282051282051282No Hit
CTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGA11.282051282051282No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCG11.282051282051282No Hit
GTCTAATTGGTCGTGGTGTTGTTTTCAGAAAAGGTTCAATTCTAGCATTT11.282051282051282No Hit
GGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAACATTGTG11.282051282051282No Hit
TGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGC11.282051282051282No Hit
GAGTAGCCCCCTCCAGGGCCTCCGCTGCCTGTGAACGCGATCCAGCCCGC11.282051282051282No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG11.282051282051282No Hit
ACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGG11.282051282051282No Hit
CTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCAT11.282051282051282No Hit
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG11.282051282051282No Hit
TCCATAATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGG11.282051282051282No Hit
CTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAGGAACATTCGAG11.282051282051282No Hit
CCACATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGT11.282051282051282No Hit
TCCCACAGCTTCTTTAACTCAAATGATCTTCTCGTCTGAATTTGTGTGTC11.282051282051282No Hit
AAACTATTTACGAACAGGTCAATGTGTAACCATTAGCGGTCATCGATTAT11.282051282051282No Hit
GAAACATCCTGAGCATGGCACCTATAATGTTCTCAAACAAAATGGCAAGA11.282051282051282No Hit
TCATTGCTCTTTGTGCAGCTGTTTGAAATTTCCCTTTGAGAATGTTGCAC11.282051282051282No Hit
CCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCG11.282051282051282No Hit
CACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGA11.282051282051282No Hit
CCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAA11.282051282051282No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC11.282051282051282No Hit
ATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGA11.282051282051282No Hit
GAATAGATTCATCGAGATTGGAGTGACAAGGAGGGAAGTTCACATATACT11.282051282051282No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.282051282051282No Hit
GGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCT11.282051282051282No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA11.282051282051282No Hit
CCTAAGAGCCTGAACAATAAGGAGCAATATTGTTGATTCAGCAGAAAATC11.282051282051282No Hit
GCTTGAAGCAGTTTGAAACGGTCTATCCCTACCAAAGAGTATCCCTCTTA11.282051282051282No Hit
AGGCAATTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTG11.282051282051282No Hit
GAGAAGACACACATTCACATCTTTTCATTCACTGTAGATGATATAGAAAC11.282051282051282No Hit
GTTAGCAAATGTTGTAAGGAAGATGATGACCAATTCCCAGGACACTGAAA11.282051282051282No Hit
TTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACT11.282051282051282No Hit
CCACCTATTAATAGTGGCCGGATTTTTTCAATTTTCTTTCTTGTTGAATC11.282051282051282No Hit
ACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGT11.282051282051282No Hit
TTGAGAGTGAGGAGAAGATTCCAAGAACTAAAAACATGAAGAAAACGAGT11.282051282051282No Hit
GATGTATATTCAGAAATGGCAGGCTGGTTTTTATAGAACCGTTGGGTGTT11.282051282051282No Hit
ACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCAAGATTGCTCAG11.282051282051282No Hit
ATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCCCATGCACCAA11.282051282051282No Hit
GTGTAGTTGCACCGGCATCATATTCCCTGCTTTTGGCTGGCCCATGGGCG11.282051282051282No Hit
ATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTG11.282051282051282No Hit
ATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACA11.282051282051282No Hit
ATTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCG11.282051282051282No Hit
GCAGTGGCGCCGCAGACAGACGATCCTTCATTACGCCGTGCAAAGGAGGG11.282051282051282No Hit
TAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATG11.282051282051282No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGA11.282051282051282No Hit
ACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGA11.282051282051282No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA11.282051282051282No Hit
GACATAATGGATCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCT11.282051282051282No Hit
GAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCATCTTGATCTTGGAG11.282051282051282No Hit
TGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTGAACAGTATATGTA11.282051282051282No Hit
ATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGG11.282051282051282No Hit
ATTGATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATC11.282051282051282No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA11.282051282051282No Hit
GGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCATGTCTCCTATTT11.282051282051282No Hit
AACCCCGGCCTGTTGTCATGCAGGACAATGGAAATGCAGGAGTTGTGCAG11.282051282051282No Hit
GGTAGACTTTGACGACTGTAAAGATATAAGCGATTTGAAGCAATATGATA11.282051282051282No Hit
AAAGAAGTGAACGCCAAAATTGAACCATTCTTGAGCACGACACCACGCCC11.282051282051282No Hit
GAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGA11.282051282051282No Hit
GTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACAATCC11.282051282051282No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT11.282051282051282No Hit
GACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCGGAGAGT11.282051282051282No Hit
GTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCT11.282051282051282No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGT50.0145.01
CTCCTTC50.0145.09
TGGTACT50.0145.04
TCTGGTA50.0145.02
GTACTCC50.0145.06
ACTCCTT50.0145.08
CTGGTAC50.0145.03
TGAATCG50.0145.0145
TACTCCT50.0145.07
GGTACTC50.0145.05
Produced by FastQC (version 0.11.5)