FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013608
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG29210.2881784674154111No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA29110.28719189272381435No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC25650.2530564083945667No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC22150.21852629418868044No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA20700.20422096116052751No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG20610.20333304393809049No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC20440.201655866962376No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT19720.19455252918287938No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA19370.19109951776229075No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG18740.18488409720523122No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA17480.17245325609111214No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT15960.15745732077884153No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG15640.1543002817657319No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC15490.1528204197283368No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC15200.14995935312270622No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC15040.1483808336161514No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC13260.13081980410572924No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA13250.13072114663656956No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA12560.12391378126455199No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT11780.11621849867009733No Hit
GTGTATGGACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATA11400.11246951484202966No Hit
ATCATAATGTGAGTCAAACCAGCCGTTGCATCATCACCATTGTTGGCTTG11030.10881918848312168No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT10910.10763529885320558No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT10780.10635275175412981No Hit
GTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGCATTTCCTGGG10580.10437960237093631No Hit
ATCCAGAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGA10550.10408362996345727No Hit
GTACTGGGCCATAAGGACCAGAAGTGGAGGAAACACTAATCAACAAAGGG10420.1028010828643815No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC10220.100827933481188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG100.00708864145.0153145
ACGGAAT203.8636912E-4108.766844
AGCGAAA1250.092.818951
GGCAGCC800.090.63457145
CTTAGGT406.113696E-790.63457145
TTAGTAG702.8085196E-972.51481
TTACGGA300.001934561572.511222
CAGGGTG702.8139766E-972.496919
ATATTCA1550.070.172154
TAGATAT1300.066.933443
GGGTAGA1750.066.299251
GTAGAAA6900.063.056351
GTTTAAA502.0082296E-458.0118451
ACATAAT2050.056.5941282
CTCCGAC400.00604838454.38074145
CGTCGGG400.00604838454.38074145
GATAGCA3750.050.274456
TACTAGT752.5482748E-548.3408162
CTGTCTA450.00963515748.338432145
ACAAGGG6700.047.6099137
Produced by FastQC (version 0.11.5)