FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTATAAGTGTC14.761904761904762No Hit
TCACCAGACACTGTAGACACAGTACTAGAAAAGAATGTAACACTAACACA14.761904761904762No Hit
GAGACTACCAGTACCAATGAACTGGAGACAGTTGAATAGATCGCAAAAAT14.761904761904762No Hit
GAATAGATAATGAAGGGGGGATACAGTAAGACTGCTGGGCGGAATGCATC14.761904761904762No Hit
ATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGATGATGACTAAT14.761904761904762No Hit
GTTGAGGATTACCTCATCTTTCAGTTTTTGTGGGTTCACTTTCAAAATGA14.761904761904762No Hit
GAAGAACATCAGACATGAGGGCAGAAATCATAAAGCGGATGGAAAGTGCA14.761904761904762No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGATGGAGAA14.761904761904762No Hit
CCTTACTGGCAAAATCTGTATTCAACAGTCTATATGCGTCTCCACAACTT14.761904761904762No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA14.761904761904762No Hit
ACATCAAAGTCATGGCGTCCCAAGGCACCCAACTGTCTTACGACCAGATG14.761904761904762No Hit
GTGTGAAATTTCCCTTTGAGAATGTTGCAAATTCTCTCATAAGCCATCCT14.761904761904762No Hit
GAATGGATCCCAGCATGTGCTCGCTAATGCAAGGTTCAACACTTCCCAGC14.761904761904762No Hit
CTACCAAAGAGTATCCCTCTTTTTCGAAGTCGTACCCACTGGCTAAGGCA14.761904761904762No Hit
CACTAGAGGAGTACAAATTGCTTCAAATGAAAACATGGATACTATGGTAT14.761904761904762No Hit
AAACTGTGTTATTGAAATAGGACTACTTCTTTGGAATAGTGATTAAGGCG14.761904761904762No Hit
CCTGTTACCCAGACGCCGGCACAGTGATGTGTGTGTGCAGAGACAATTGG14.761904761904762No Hit
GCAGTTCTCCGATAAAAGATCCTTAGACCAGTACCTTCTGGCATGAGGTC14.761904761904762No Hit
ATAATAGTAGAGCCTGAAGACCAAAACGCACTTTTGAAGCACAGATTTGA14.761904761904762No Hit
CTCTTGCACCTGTAATTTCAATCTTTGGAGAATGCCGGGAAGCACTTCTT14.761904761904762No Hit
GTAAGTAGTATTGGTGTATCTCTTTTGTACAAGATTCAGCATGGAGACGC14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)