FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180200
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT30970.2624131503135062No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA26430.22394509405185564No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC24090.20411794611082867No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT23060.19539061176071854No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC22670.1920860871038807No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG22570.1912387730893069No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT22150.18768005422809694No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG21620.1831892899508558No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC21140.17912218268090155No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA20410.1729367903745128No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC19940.16895441450601592No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA19140.16217590238942553No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT18470.15649889849178106No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG18470.15649889849178106No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC18190.15412641925097442No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC18170.15395695644805965No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA18080.15319437383494325No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC17300.14658532452126757No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC17240.14607693611252331No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG16430.1392136925944755No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG16410.13904422979156075No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA16400.13895949839010335No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG15390.13040162684290799No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT14960.12675817658024063No Hit
CGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCACAATATCAAGTG14230.12057278427385189No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC14100.11947127605490594No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG14030.11887815624470428No Hit
CCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG13750.11650567700389763No Hit
CAACAAGAGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTG13730.11633621420098289No Hit
GTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG13730.11633621420098289No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC13650.11565836298932383No Hit
CTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGAGA13580.11506524317912217No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA13210.11193018132519912No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAGGCA13050.11057447890188105No Hit
CCACAATATCAAGTGCAAAATCCCAATGATACTTGCGGCAACAACAAGAG12950.10972716488730724No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACA12740.10794780545670225No Hit
CTCATGGAATGGCTAAAGACAAGACCGATCCTGTCACCTCTGACTAAGGG12700.10760887985087272No Hit
CTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGA12400.10506693780715133No Hit
GGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT12330.10447381799694967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG100.007087399145.0248145
GTTACGC100.0070882966145.018663
AGCGAAA900.0112.792281
TAGGGGC700.093.230225145
AACCGCG259.380798E-487.01487145
GCATATA259.380798E-487.01487145
AGGACGC353.434621E-582.87131145
TTAGTAG809.822543E-1172.509331
ATATTCA3650.065.556374
GTAGAAA7450.062.2898861
GGGTAGA1300.061.354041
ACTAATA400.00605111954.375088
TTATATT4450.053.770962
CAAGGTA4550.052.5824939
TAGATAT3500.051.7923743
GTCAATT1004.699541E-850.7565272
AAGACTG450.00963305548.3416145
GCTAAAC450.00963305548.3416145
CTTAGGT450.00963305548.3416145
AGGGTGT2750.047.4626586
Produced by FastQC (version 0.11.5)