FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAAT30.26714158504007124No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT30.26714158504007124No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.26714158504007124No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT30.26714158504007124No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.26714158504007124No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG30.26714158504007124No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA30.26714158504007124No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT20.17809439002671415No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA20.17809439002671415No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG20.17809439002671415No Hit
GTCTTGCCATTTTGTTTGAGAACATTATGGGTGCCATGCTCAGGATGTTT20.17809439002671415No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA20.17809439002671415No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT20.17809439002671415No Hit
CTAGAGATCTGGGCTCTGGCTCATCACTGTCATACTGTTTAAGGTCTCCA20.17809439002671415No Hit
CTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACACT20.17809439002671415No Hit
CATTTGGTCTCATCAGGCTGACCACTTGGCTGTTTTGGAGTAATTTGAAT20.17809439002671415No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20.17809439002671415No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20.17809439002671415No Hit
CCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCAT20.17809439002671415No Hit
GGGTTAAGTGCAACACTTCAATATAAACACTGCCTGTTCCGCCGGCTACT20.17809439002671415No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC20.17809439002671415No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA20.17809439002671415No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA20.17809439002671415No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC20.17809439002671415No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT20.17809439002671415No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA20.17809439002671415No Hit
CTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTG20.17809439002671415No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.17809439002671415No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA20.17809439002671415No Hit
AACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATG20.17809439002671415No Hit
AACCCAATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTAT20.17809439002671415No Hit
CCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGT20.17809439002671415No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20.17809439002671415No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.17809439002671415No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT20.17809439002671415No Hit
GTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTG20.17809439002671415No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20.17809439002671415No Hit
TCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGA20.17809439002671415No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT20.17809439002671415No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20.17809439002671415No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT20.17809439002671415No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC20.17809439002671415No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT20.17809439002671415No Hit
GTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAGATTACAGGAA20.17809439002671415No Hit
GTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAA20.17809439002671415No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCAC20.17809439002671415No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT20.17809439002671415No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC20.17809439002671415No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA20.17809439002671415No Hit
GCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCC20.17809439002671415No Hit
ACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTG20.17809439002671415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT200.00557080229.0120-124
Produced by FastQC (version 0.11.5)