FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT25.405405405405405No Hit
GTTTGTCCTCTGTTTCTTATAGCTGTAGTCATTTCTATTTTGCACTTTGT12.7027027027027026No Hit
ACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATGATCTTCT12.7027027027027026No Hit
GAAGAATACTGGGAAATTTAAAAATGCATGGGGAGGGACGCTTGGGTGGC12.7027027027027026No Hit
AAGTAAAATCATACCTACCATGATAAATCTCTAGCTATTACTGCAAAGCC12.7027027027027026No Hit
AAAGAAGTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTT12.7027027027027026No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG12.7027027027027026No Hit
GAAATTTTCCTTTTACGATATTGCACATTATTTCATAAGCAAAACTTGTC12.7027027027027026No Hit
GTCCAAGATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAAC12.7027027027027026No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC12.7027027027027026No Hit
GCAGAGTGTGTTGGGCACAAGGCATTTGCGGTGCTGCCTTCCTCTCCTTT12.7027027027027026No Hit
ACGTGCGTCAGCACAGATGACCCTTGAGGATATTATTAAGTCAAATAAGC12.7027027027027026No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT12.7027027027027026No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT12.7027027027027026No Hit
GAGAAATATTTCATCGGGGTAGAAAATAACAGGTTGCAGCTTTACTGCTG12.7027027027027026No Hit
AGTAAAACATGTAAGGAAAATTTTAGGGATGATAAAGAAAAAAATAACAC12.7027027027027026No Hit
CAGTTTGGGGGAGGGGAAATCAGGAGTTCACTTTGGAGATGTAGAGTCTG12.7027027027027026No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA12.7027027027027026No Hit
CCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAAT12.7027027027027026No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA12.7027027027027026No Hit
TAACTCCAGTCCCACCGGACTGCATATGTAAGGGCAGAATGTGACATCAC12.7027027027027026No Hit
CTATATGAACTGCTAGGGAAAATTTTATCGAATAGATTGCAGCACTTCTG12.7027027027027026No Hit
ATATTATACATGAGAAAACAGGCTTGGAGAGGAGCATTCGAGAATCCCAT12.7027027027027026No Hit
GAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATA12.7027027027027026No Hit
GGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAAC12.7027027027027026No Hit
GGCGGGGGCTGTGGATGTGGCGGAGTCTGCGACGGCTCGCTCGCCCTCGC12.7027027027027026No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA12.7027027027027026No Hit
GGGTGTGTTCATGATGGAGACGTTAACTTCATTATTTTTGAAGTCTGAGT12.7027027027027026No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT12.7027027027027026No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA12.7027027027027026No Hit
GGCCTCTGCCTCCCTCCGGGCCTCGCAGGAGGAGGTGTAGGGGTTGTGGG12.7027027027027026No Hit
CCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGT12.7027027027027026No Hit
AACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGT12.7027027027027026No Hit
GTCTGTGCATGCTTTGGCAGGCTCCATAATCCAGGACCTCTCCGTGGGTC12.7027027027027026No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG12.7027027027027026No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAG12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)