FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCCGCCTATATGATGCTCTCAATCTCCTGAAGAGAGTGAAGCCGGGG14.761904761904762No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTGC14.761904761904762No Hit
GCTTTGATCAACATCATCATTCCTCACTTCTCCACCTGGAGTGTACATCT14.761904761904762No Hit
GGTTGTCCCTAAGTGCCTGAACAATGAGAAGCTGGGTGCCTCTCCGATTG14.761904761904762No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG14.761904761904762No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG14.761904761904762No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCCCTAAGACCACTGT14.761904761904762No Hit
CCTAGATGAACTGCTAGTGAAAAATTTATCGAATAGATTTCAGAACTTAT14.761904761904762No Hit
CACTAGACCAAAAGAAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA14.761904761904762No Hit
GAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACA14.761904761904762No Hit
AAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGG14.761904761904762No Hit
CATGTAAGCTGATTCATTTACTAAAGACACTAAAAATCTGGGTAGAGAAA14.761904761904762No Hit
ATTTGAACCATTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACA14.761904761904762No Hit
AGTAGAAACAAGGTCGTCTTTAAACAATTCGACACTAATTGATGGCCATC14.761904761904762No Hit
CATTAACACAAAAGATCTACTCCTCTGAATTTTACTGACACCCCTAAAGC14.761904761904762No Hit
CTACTTTTCCTAAAATGCCATAAGCACCACATTCCCTTATACTGGAGATA14.761904761904762No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT14.761904761904762No Hit
CAGTAAACAGAACACACCAATACAGATGTACCAGAAGTGCTGCAATCTAT14.761904761904762No Hit
AAGTTCACCACATCAGTATCATTTCTCAAATGAGACCTTCCTATTATAAT14.761904761904762No Hit
AATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTT14.761904761904762No Hit
GAGTGTACATAAATACGGCCTTGCTCAATGACTCCTGTGCAGCCATCGAT14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)