FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998309
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT16400.16427779374922996No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA16050.16077186522409392No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG15070.15095526535371312No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA11690.11709801273954257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.049.0452841
CGAAAGC4300.040.466083
GCGAAAG4700.037.0221632
TAAGGCG900.00362780532.2229924
GTATAGG3500.031.072171
TATACAG12200.026.1481655
TATACGT1409.993187E-425.8934754
ATATACT10700.025.0706924
GTATATA22700.024.5930981
TATAGAC5050.024.4065232
ATACTTA12150.022.6754386
TATATAT11550.022.597942
TATACTT12850.022.5686325
TAAACGT2252.6510486E-522.5560934
TATATGT10500.022.0957644
TATATAC24300.022.0787163
GTGTAAG8900.021.994911
CAACCGC1650.002600496621.970226
TACTTAC11550.021.969127
CGTGTAT2353.7099213E-521.5951798
Produced by FastQC (version 0.11.5)