FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGC30.1756440281030445No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA30.1756440281030445No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20.117096018735363No Hit
CTACCATCTGAGTCAGCCGCGCACCCCTGAAAGTTATTGTTACAAGGAAA20.117096018735363No Hit
CTGCAAAGCCCGAGAGTTGGGCTTAAATTTGAATGAGGTTGTACTGAATC20.117096018735363No Hit
GGGCAAGAGTCCCACGCTCTACCATCTGAGTCAGCCGCGCACCCCTGAAA20.117096018735363No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA20.117096018735363No Hit
ATCTTCTTTGCCCAGAATGAGGAATCCCCTTAGAACAGCGGATTCCACTC20.117096018735363No Hit
CTCCTGTATGAAGCCATGGGCATTAGCTGCCTGGGCAGCTGCCATCACCT20.117096018735363No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG20.117096018735363No Hit
AAACAAGGGTATTTTTCATTAATTGTCGTACTCCTCTGCATTGTCTCCGA20.117096018735363No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG20.117096018735363No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT20.117096018735363No Hit
TATCTACTCTAGGCTTTCTAAAAACTTTTTCATCAAATCAAAAATAAAAG20.117096018735363No Hit
AGGCAGCAGAGGCCATGGAGGTTGCTAGCCAGGCCAGGCAGATGGTGCAG20.117096018735363No Hit
GAATCCAGTGATAAACAAAAGAAAGCCCTCACTTTCATGTTCTAGGGACA20.117096018735363No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA20.117096018735363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG100.0064843297145.02
GTTTTGG100.0064843297145.01
Produced by FastQC (version 0.11.5)