FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865662
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA10480.12106341736151062No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG9350.10800982369562255No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT9290.10731671252752228No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCT300.001934446372.5114064
AGCGAAA2400.039.277011
GTATAGG2455.4569682E-1132.556141
GTACTAC1204.0538612E-430.2130851
CGGTTCG1453.486112E-530.004721
CGAAAGC4150.029.7034683
ATATACT7800.027.8890044
GCGAAAG4700.027.7703272
GTTACGC3453.6379788E-1227.323143
GTATATA12600.027.0479051
TATACTT8800.026.3677865
TATACGT1658.408283E-526.3677854
CTACGCC3057.0940587E-1026.1516573
CGACCAA1958.74203E-626.0297375
TATAATA5050.025.8456522
GGTTCGC1701.0300335E-425.5922622
TTACGCA3709.094947E-1225.4769824
TATATAC14750.025.071743
TAGAAAC8650.024.3101842
TCGCAGT2101.5528612E-524.1704715
Produced by FastQC (version 0.11.5)