FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATGATCTTCT12.941176470588235No Hit
AATTTTCACAATTTCCCAGTTCCTGATTATCCATTGATAAGTGTTGACTA12.941176470588235No Hit
ATGTTGACCAAAGTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCA12.941176470588235No Hit
CTTCAGGAATGGGAAGATTCCGTATATTTTATAATGTTTGTACTCCATCT12.941176470588235No Hit
GCTGGGAGCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTT12.941176470588235No Hit
ATATTATTGTGTCCCCGGGTTCCAGCAGAGTCCAGTAGTAGTTTATTGTT12.941176470588235No Hit
CTTTTGTTATTGCCAGGTGTGACTCAGATGACAGTATTCTTCCCACCACA12.941176470588235No Hit
GAAAGAAGAGCAGCTCTGTCAGCAGACCCATTACCATCTCTCATGGAAAT12.941176470588235No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCAAGATAA12.941176470588235No Hit
ATATATGAGAGAAACAAATTACCCTCCAAGCACAATTTGAAATCGAGCAA12.941176470588235No Hit
GAATGAAGATTCGAACACAAATACCAGCAGAAATGCTAGCAAGCATTGAC12.941176470588235No Hit
GATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCCCATGCACCA12.941176470588235No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTATTGGGTC12.941176470588235No Hit
GGCATGGAAATGAGGCGCTGCCTTCTTCAGTCTCTTCAGCAGATTGAGAG12.941176470588235No Hit
GAGATCAAACAGAGTCCGAACAGGTCAATCGCGAACAAGTCAGAGAAACT12.941176470588235No Hit
CCTCAAGACGGCCTTGCTCAATGACTCCTGTGAAGACATGGATTCCTTTC12.941176470588235No Hit
CTTCATTTCAATGCTTGCTAGCATTTATGCTGGTATTTTTTTTAGAATCT12.941176470588235No Hit
CAGTAAACAGAACACACCAATACAGATGTACCAGAAGTGCTGCAATCTAT12.941176470588235No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA12.941176470588235No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA12.941176470588235No Hit
GGTCGGGATTAGTAGCTGGACATCGGGAGAGAAGGGGAAGGTTGGTCCGT12.941176470588235No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAAGCTCAGCGTTT12.941176470588235No Hit
ATTATAAACAATGAAGACATATACACATAAAGTGCAAATTTGTTACACAA12.941176470588235No Hit
CAACAGGATGGGGGCTGTGACCCCCGAATCGGCATTTGGACTAATATGCG12.941176470588235No Hit
CCAGAAATCAGCCTGAATGGTTCAGAAATATTCTAAGTATTGCTCCAATA12.941176470588235No Hit
CACTGATTCAATCTCTCTATGACTGACAAATGCGTTCAATCGGTTGCATA12.941176470588235No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG12.941176470588235No Hit
CTGCTGCATTTGAAGATTTAAGAGTATCAAGTTTCATAAGAGGAAAGAAA12.941176470588235No Hit
CCACAAATGCGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATG12.941176470588235No Hit
ATTAAGATCAATCCCATCACATTGTCGATGGACACAAGTCACAAGATCTC12.941176470588235No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC12.941176470588235No Hit
GTAAACAGAACACACCAATACTCAGAAAAGGGAAACTGGACGACAAACAC12.941176470588235No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA12.941176470588235No Hit
TCATTAATCAGGCAATACTCGATTGCTTCATATAGCCCCCCAAGATCTAA12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)