FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975183
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT14050.14407552223531378No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC12690.13012942186235815No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA11870.12172074369631136No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA10870.11146625812796163No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT10410.10674919476652074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA100.0070915106144.995387
CTATCGC203.864152E-4108.76327145
AGCGAAA1150.094.5816041
GACTACC502.009007E-458.00708145
ACTATAC752.548698E-548.339233
GGTCGTT1203.621608E-948.33187
CAAGGTA2600.044.620834
TATAGAC657.332084E-444.620832
GGATAGC3300.041.7475175
TAGCCCG1053.8208163E-641.4336285
GGGTAGA1807.2759576E-1140.284761
GTCGTTT1553.4711775E-837.4181638
GATAGCA3800.036.2544256
GTAGAAA6850.035.991641
TAGATAT3200.033.988523
GTAGATA3600.032.2261542
TAGCTCT7750.031.8103354
ATATAGT6450.031.4783211
GTATTAA5000.030.4552761
CAATTCG3350.030.302204145
Produced by FastQC (version 0.11.5)