FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACTATGCCAACGAGCAGCTTCAGTATTATTTCAACCAGCACATTTTCA11.2658227848101267No Hit
CTGGTTACAGGTCCTATCTCTGACGTGTGGGTCATATCCATGACTGTACG11.2658227848101267No Hit
ATATGGATCAGCCACTCAATCCAAACTGGAAGTCAAAACTACACTGGAAT11.2658227848101267No Hit
GCATAGACTTGGGCCTTTCTGGATGAGCATTTTCAAAAAACTGGATGCCC11.2658227848101267No Hit
AAGATGATCTTGGGACTTGCTGTGTTCCAGGAAAAGAAAGAGAAGAATAA11.2658227848101267No Hit
CCATACAGCCATGGAACACCTACAGCATACACCATGGTCACAGTAAACAC11.2658227848101267No Hit
CTCTCGAAGTCTTCAAATCCGAATATGTCGAGGACCCCAATGGACAAGCA11.2658227848101267No Hit
GCTCGGCAGGGAGCCTGCTTCCCTTCCCCTCTCTCTCTGCCTGCCTCTCT11.2658227848101267No Hit
CCATAGAAGACACCGGGTACATGCTTAAGGAGGGGTGAGCGATACCCACA11.2658227848101267No Hit
CTATAGTGTTGGCCAATGCTGTAGCAGCAGGCTGGTTTCTATTTAGAGTC11.2658227848101267No Hit
GCAGCAGACAGAGGAGGACAGAGAAGCAGGGAGTTTGGAAACTCCGCAGA11.2658227848101267No Hit
CTAGAAGAGAATACCGTTTGCCCCTTGATGCTCAAAGAGCTCTGCAGAAA11.2658227848101267No Hit
ATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAGATATGCATTCGC11.2658227848101267No Hit
GATAACAGGCTCCTGCTCCACCGAAGTCACCTGTCGATGCAGGTAGCAGT11.2658227848101267No Hit
GGGTGGTGTTTCCCCACAGATGGTCACAGTGCTGTTGTTTTTCAGGGGAG11.2658227848101267No Hit
ATCTCAATTCATGTGTTAGGATGAAGTTGAGCCAAGAAGCATTAAGCAAA11.2658227848101267No Hit
AACTATGCCAACGAGCAGCTTCAGTATTATTTCAACCAGCACATTTTCAA11.2658227848101267No Hit
GGAATTACTTCACAGCAGAGGTGTCTCATTGCAGAGCCACAGAATATATA11.2658227848101267No Hit
ATTCAATACTATTTGTTGATGGAATAAATGAATTTCTAATTTTTCTTCTT11.2658227848101267No Hit
GGGTAGATAAACTAACTCAAGGGCGCCAGACTTATGATTGGACATTAAAC11.2658227848101267No Hit
ACCTTAGACTTAGCCACTCTGGACACATAGACATGCATGTGGTGGGCCTG11.2658227848101267No Hit
GTCATAAAGGATGACTTCTCTCCACCACTTTCCGGCTGCTACTCTATTTA11.2658227848101267No Hit
GAAGAGGTCCATGACCTCAAAAAAGATCAAATGAACAACTGCATTATTTT11.2658227848101267No Hit
TCTCAGTTCCATGCGTATCACTGACTTCTTCGCGAGACAATATTACGAAC11.2658227848101267No Hit
GGCAAATGTTGTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTT11.2658227848101267No Hit
GGCTGCTGGGTAAGCAGAGAGCCGGCTGTAGGGCTCCAAGCCAGGACCCG11.2658227848101267No Hit
CAACCAAGTTAAAGGCATTTTAGGCCACCAGGAAATTGTCCTCATCCAAG11.2658227848101267No Hit
GCGATGAGATCAATATTAAAATTTACTTGTAAAGACTAAAAAGTTTATCT11.2658227848101267No Hit
CAAGGAATGTGGAAATGATAAAGAATGAGCAAATGCTGAGTGGGTGTCTT11.2658227848101267No Hit
TTATTAAGTTCCGAAGCTCCTCCTTTACCTGGAAAGCTTTGATATATCTT11.2658227848101267No Hit
GTTGTGCAGAAGCAGTGGGGGCCAGCTGGTGTCGCGCAGGAAGTTGGCTC11.2658227848101267No Hit
CTCCCTCAACAATCACAAGCAATTTACTAAACATCCAAATGTAAGCATAT11.2658227848101267No Hit
GTGTTCGCTGTGCGGGCATGAGGCGCAGGTGTAAGAAAAGCGGGTGTTAA11.2658227848101267No Hit
CCCCAGTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAG11.2658227848101267No Hit
GGTGCATGCTCATGCCCCAGCAGACAGTGGCCGGGCCGCGTTGGGTCAGG11.2658227848101267No Hit
GTAATCTTCAGGCGGCCAGCGTGGAATTACCTGGGTCACCAGGGCTGACC11.2658227848101267No Hit
CATGAAAAAGGAGCAGTCAGACATCAGGAAAGACTACTTTGGGGAGAAAG11.2658227848101267No Hit
CCATTTACTCCTCTGCAGAGAGGAAAGCTGCTCTCCTTGCTTAGGGAGTC11.2658227848101267No Hit
GCTCAGTGGGTTGAGCCTCTGCTTTTGGCTCCAGTCATGATCTCAGGGTC11.2658227848101267No Hit
GTCATCCTCCAGGAGATCAACCAAGACACAGTCCTTGAAAATATTGTTCT11.2658227848101267No Hit
GTCCGTGACAGAGGCAGCCATCGCCGGGCTCAACAACGAGCAGGAAGACA11.2658227848101267No Hit
GAACTGGATCAGGTGCAGGAATAGGAGCAAGAACAGGAGCAGGTGCAGAA11.2658227848101267No Hit
AGCTTTCGGAATTCTAGAGTACAAGTTGATAGGCCAATGGTAATCGAGGT11.2658227848101267No Hit
GTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTCCGCAGCGTGAAGG11.2658227848101267No Hit
GGGGAGAAAACAGGCTTCAAGTGACTTGCCTAAGGCAATCTCTAGATAGT11.2658227848101267No Hit
GGGAGACAAAAATCACCAGTGAACTGGCGACAGTTGAGTAGATCGCCAGC11.2658227848101267No Hit
GAGTAAGTTATTGTTAGTTTCTCTGTTCCCTGTGTTTCACTGACCTCCTC11.2658227848101267No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC11.2658227848101267No Hit
CATCAGAGATGCCTATGTACGCTGAAGTTCAAAAACTACTAGCCAGAGGA11.2658227848101267No Hit
AGTTAAACGCTGTTTCCTAAGTTCAACCCGAAACCTAGCATAGGAACAGA11.2658227848101267No Hit
CCCCCAGTCACTCGGCCTCTGGGCTTGCACAAGACCTGGGAAGGCGGTGC11.2658227848101267No Hit
TACCATATTTGCCCCGGGTTGGCGCATGCAGGTCATATGAGGCAGAGTCG11.2658227848101267No Hit
GCTGTACAGCTGGTAAAGACAACCCCTTTGAAGCCCTCGCCTCTGCCTGT11.2658227848101267No Hit
GCATCTGTGTGTTCGTTAGGGAGGCACAGGCTCACCTAGAAACCCTGGCG11.2658227848101267No Hit
TCCTGGCGCCGTCGCAGAGGCAGCGTGTGCCCCGGGCCTGGAACACCAGC11.2658227848101267No Hit
GCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCCGCTTGTCCTTT11.2658227848101267No Hit
AATGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAAAAGAC11.2658227848101267No Hit
CTTTAGAAGAGCTTCTAAAATATGCAAATATATTTCTATACTCCAATGTA11.2658227848101267No Hit
GCTCAGGTCATGATCCCAGGGTCCTGAGATCAGACCCACATCAGGCCTCC11.2658227848101267No Hit
CTTATACCATATACGGAATCTTCACATTCATGAAGGCTGCTTAAAATGGG11.2658227848101267No Hit
TTACTGTCCTACGTAAGGGCTTCCGGAAGGCTAAACCTGCTGGCAGGAAA11.2658227848101267No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG11.2658227848101267No Hit
TCCCATCACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACTT11.2658227848101267No Hit
CAGTTACTGCGTGAAGGTAAAAGTCAACCGACAGCAGCTGGTCAAGCTGG11.2658227848101267No Hit
CAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCA11.2658227848101267No Hit
AGTTTATATTCGGTTATTTTCTTAAGTACTGTGTATTAAGTTAAATTTGG11.2658227848101267No Hit
CCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCATTGATAAGTGT11.2658227848101267No Hit
CCATAGAACTTGAAGAAATAGCAGAATATCATTAAACGATTGAACAAATC11.2658227848101267No Hit
CTCAGTGACAGACAGCCATCATTGTCGTCAATCCATACAGCGCACAACTA11.2658227848101267No Hit
GTGATGATATCACCCCTGGCTGTAACATGGTGGAATAGAAATGGACCAGT11.2658227848101267No Hit
GTATCTTACAGAGTCGTGTTTTTTGGATAAAATACCAAGTTTCACCGTTT11.2658227848101267No Hit
GAGCCAGCGTTCATCATCCAGCACTTCGCCGGGAAAGTGAAATACCAGAT11.2658227848101267No Hit
GGCACATATCGGGCAAACCACAGATTTGGTATCCGTGCTATGGGATAACT11.2658227848101267No Hit
GTTATGTCCCATTTTTTTTTTATTTCCAGTGTAACTATGAGAAGAGAAAA11.2658227848101267No Hit
CAGTGCCCAGTGTGCAGCTCGGGAAGGCCCCTCGTCGTCTGCAGGGGCCT11.2658227848101267No Hit
GGCGTGGACAGTGCTGAACAGTATATGTAACACATCAGGGGTAGAGAAGC11.2658227848101267No Hit
GGAAAAGGACTAAATATGATAATGCCTGAAAAACATTTAGCATACTTCCT11.2658227848101267No Hit
TTGTACTCCTCTAGTGGAAAGCTTCCCCCTTGGAAGTACTCTTGTCCCTC11.2658227848101267No Hit
GGGCGATGCGGAGATATCAGAGGTGGAAGCGCCCACACCCCCTGGAGAGG11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAC50.0145.03
ACAGAGG50.0145.08
GAAACAC50.0145.0145
GACAGAG50.0145.07
GTCCGTG50.0145.01
TGACAGA50.0145.06
CAGAGGC50.0145.09
GTGACAG50.0145.05
TCCGTGA50.0145.02
CGTGACA50.0145.04
Produced by FastQC (version 0.11.5)